ELISA/assay

Human Splicing factor 3A subunit 3, SF3A3 ELISA Kit

MBS2602705

48-Strip-Wells

275 USD

ELISA Kit

Human Splicing factor 3A subunit 3, SF3A3 ELISA Kit

Splicing factor 3A subunit 3, SF3A3

splicing factor 3A subunit 3; Splicing factor 3A subunit 3; splicing factor 3A subunit 3; SAP 61; spliceosome associated protein 61; spliceosome-associated protein 61; splicing factor 3a, subunit 3, 60kD; pre-mRNA splicing factor SF3a (60kD); splicing factor 3a, subunit 3, 60kDa; SF3a60; Spliceosome-associated protein 61; SAP 61

SF3A3

SF3A3; SF3A3; PRP9; PRPF9; SAP61; SF3a60; SAP61; SAP 61

SF3A3_HUMAN

Human

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant SF3A3 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human SF3A3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

5803167

NP_006793.1

NM_006802.2

Q12874

58,849 Da

Diurnally Regulated Genes With Circadian Orthologs Pathway (198813); Gene Expression Pathway (105937); Processing Of Capped Intron-Containing Pre-mRNA Pathway (160950); Spliceosome Pathway (125136); Spliceosome Pathway (124832); Spliceosome, U2-snRNP Pathway (413436); MRNA Splicing Pathway (105951); MRNA Splicing - Major Pathway (105952); MRNA Processing Pathway (198843)

This gene encodes subunit 3 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 3 interacts with subunit 1 through its amino-terminus while the zinc finger domain of subunit 3 plays a role in its binding to the 15S U2 snRNP. This gene has a pseudogene on chromosome 20. [provided by RefSeq, Jul 2008]

SF3A3: subunit of the splicing factor SF3A required for A complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the E complex. Protein type: Spliceosome; RNA-binding; Motility/polarity/chemotaxis; RNA splicing. Chromosomal Location of Human Ortholog: 1p34.3. Cellular Component: nucleoplasm; spliceosome; nuclear speck. Molecular Function: protein binding; zinc ion binding. Biological Process: nuclear mRNA 3 -splice site recognition; RNA splicing, via transesterification reactions; nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression; mRNA processing

48-Strip-Wells

275 USD

96-Strip-Wells

420