catalog number :
MBS2602703
products type :
ELISA Kit
products full name :
Human E1A-binding protein p400, EP400 ELISA Kit
products short name :
E1A-binding protein p400, EP400
other names :
E1A-binding protein p400; E1A-binding protein p400; E1A-binding protein p400; hDomino; domino homolog; CAG repeat protein 32; p400 SWI2/SNF2-related protein; p400 kDa SWI2/SNF2-related protein; trinucleotide repeat containing 12; trinucleotide repeat-containing gene 12 protein; E1A binding protein p400; CAG repeat protein 32; Domino homolog; hDomino; Trinucleotide repeat-containing gene 12 protein; p400 kDa SWI2/SNF2-related protein
products gene name :
EP400
other gene names :
EP400; EP400; P400; CAGH32; TNRC12; CAGH32; KIAA1498; KIAA1818; TNRC12; hDomino
uniprot entry name :
EP400_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant EP400 concentration. Assay Type: Sandwich. Detection Range: 100 ng/ml-1.56 ng/ml. Sensitivity: 0.5 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human EP400 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_056224.3
ncbi gb acc num :
NM_015409.4
ncbi mol weight :
343,489 Da
uniprot summary :
p400: Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. May be required for transcriptional activation of E2F1 and MYC target genes during cellular proliferation. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. May regulate ZNF42 transcription activity. Belongs to the SNF2/RAD54 helicase family. SWR1 subfamily. 5 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.6.1.-; EC 3.6.4.-; Apoptosis; Helicase. Chromosomal Location of Human Ortholog: 12q24.33. Cellular Component: nucleoplasm; NuA4 histone acetyltransferase complex; nuclear speck. Molecular Function: DNA binding; chromatin binding; helicase activity; ATP binding. Biological Process: establishment and/or maintenance of chromatin architecture