ELISA/assay

Mouse Nuclear factor of activated T-cells, cytoplasmic 3, NFATC3 ELISA Kit

MBS2602702

48-Strip-Wells

290 USD

ELISA Kit

Mouse Nuclear factor of activated T-cells, cytoplasmic 3, NFATC3 ELISA Kit

Nuclear factor of activated T-cells, cytoplasmic 3, NFATC3

Nuclear factor of activated T-cells, cytoplasmic 3; Nuclear factor of activated T-cells, cytoplasmic 3; nuclear factor of activated T-cells, cytoplasmic 3; NF-AT4; NF-ATc3; T-cell transcription factor NFAT4; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3; nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3; NFATx; T-cell transcription factor NFAT4; NF-AT4

NFATC3

Nfatc3; Nfatc3; NFAT4; NFATx; C80703; D8Ertd281e; Nfat4; NF-ATc3; NFATc3; NF-AT4

NFAC3_MOUSE

Mouse

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant NFATC3 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse NFATC3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

9087154

P97305.1

P97305

115,451 Da

Axon Guidance Pathway (83262); Axon Guidance Pathway (476); B Cell Receptor Signaling Pathway (198285); B Cell Receptor Signaling Pathway (83278); B Cell Receptor Signaling Pathway (492); FCERI Mediated Ca+2 Mobilization Pathway (970716); Fc Epsilon Receptor (FCERI) Signaling Pathway (970714); HTLV-I Infection Pathway (373903); HTLV-I Infection Pathway (373889); Heart Development Pathway (672437)

NFAT4: a transcription factor that plays a role in the inducible expression of cytokine genes in T cells. Plays a role in the regulation of gene expression in T cells and immature thymocytes. Six spliced isoforms have been identified. Isoform 1 is predominantly expressed in thymus, peripheral blood leukocytes and kidney. Isoform 2 is predominantly expressed in skeletal muscle and is also found in thymus, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta and pancreas. Isoform 3 is expressed in thymus and kidney. Isoform 4 is expressed in thymus and skeletal muscle. Protein type: DNA-binding; Transcription factor. Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus; cytosol. Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; chromatin binding; transcription factor activity; transcription factor binding. Biological Process: cardiac muscle hypertrophy; transcription from RNA polymerase II promoter; transcription, DNA-dependent; thymus development; heart development; positive regulation of transcription, DNA-dependent; cellular respiration; myotube differentiation; muscle cell development; myotube cell development; skeletal muscle fiber development; patterning of blood vessels; calcineurin-NFAT signaling pathway; regulation of transcription, DNA-dependent; response to hypoxia; smooth muscle cell differentiation; blood vessel remodeling; positive regulation of transcription from RNA polymerase II promoter; T cell differentiation

48-Strip-Wells

290 USD

96-Strip-Wells

450