catalog number :
MBS2602701
products type :
ELISA Kit
products full name :
Rabbit ADAM metallopeptidase with thrombospondin type 1 motif, 13, ADAMTS13 ELISA Kit
products short name :
ADAM metallopeptidase with thrombospondin type 1 motif, 13, ADAMTS13
other names :
A disintegrin and metalloproteinase with thrombospondin motifs 13 isoform 1; A disintegrin and metalloproteinase with thrombospondin motifs 13; A disintegrin and metalloproteinase with thrombospondin motifs 13; vWF-cleaving protease; von Willebrand factor-cleaving protease; vWF-CP mRNA for von Willebrand factor-cleaving; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 13; a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 13; von Willebrand factor-cleaving protease; vWF-CP; vWF-cleaving protease
products gene name :
ADAMTS13
other gene names :
Adamts13; Adamts13; Gm710; vWF-CP; ADAM-TS13; ADAMTS-13; Gm710; ADAM-TS 13; ADAM-TS13; ADAMTS-13; vWF-CP; vWF-cleaving protease
uniprot entry name :
ATS13_MOUSE
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Rabbit serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant ADAMTS13 concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rabbit ADAMTS13 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_001001322.1
ncbi gb acc num :
NM_001001322.2
ncbi mol weight :
155,357 Da
ncbi pathways :
Metabolism Of Proteins Pathway (970583); O-glycosylation Of TSR Domain-containing Proteins Pathway (1000404); O-linked Glycosylation Pathway (1000403); Post-translational Protein Modification Pathway (1000373)
uniprot summary :
ADAMTS13: Cleaves the vWF multimers in plasma into smaller forms. Defects in ADAMTS13 are the cause of thrombotic thrombocytopenic purpura congenital (TTP); also known as Upshaw-Schulman syndrome (USS). A hematologic disease characterized by hemolytic anemia with fragmentation of erythrocytes, thrombocytopenia, diffuse and non-focal neurologic findings, decreased renal function and fever. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Extracellular matrix; Secreted, signal peptide; Protease; Secreted; Calcium-binding; EC 3.4.24.87. Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region. Molecular Function: peptidase activity; hydrolase activity; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; metal ion binding; endopeptidase activity. Biological Process: hemostasis; peptide catabolic process; blood coagulation; proteolysis