ELISA/assay

Human Small ubiquitin-related modifier 3, SUMO3 ELISA Kit

MBS2602699

48-Strip-Wells

275 USD

ELISA Kit

Human Small ubiquitin-related modifier 3, SUMO3 ELISA Kit

Small ubiquitin-related modifier 3, SUMO3

small ubiquitin-related modifier 3 isoform 2; Small ubiquitin-related modifier 3; small ubiquitin-related modifier 3; ubiquitin-like protein SMT3B; SMT3 suppressor of mif two 3 homolog 1; SMT3 suppressor of mif two 3 homolog 3; small ubiquitin-like modifier 3; SMT3 homolog 1; SUMO-2; Ubiquitin-like protein SMT3B; Smt3B

SUMO3

SUMO3; SUMO3; SMT3A; Smt3B; SMT3H1; SUMO-3; SMT3B; SMT3H1; SUMO-3; Smt3B

SUMO3_HUMAN

Human

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant SUMO3 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human SUMO3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

556695502

NP_001273345.1

NM_001286416.1

P55854

11,637 Da

Diurnally Regulated Genes With Circadian Orthologs Pathway (198813); Metabolism Of Proteins Pathway (106230); Nuclear Pore Complex Pathway (470351); Nuclear Pore Complex Pathway (890636); Post-translational Protein Modification Pathway (161012); Processing And Activation Of SUMO Pathway (833806); RNA Transport Pathway (177876); RNA Transport Pathway (175229); SUMO Is Conjugated To E1 (UBA2:SAE1) Pathway (833808); SUMO Is Proteolytically Processed Pathway (833807)

This gene encodes a member of the small ubiquitin-related modifier (SUMO) family of eukaryotic proteins. The encoded protein is covalently conjugated to other proteins via a post-translation modification known as sumoylation. Sumoylation may play a role in a wide variety of cellular processes, including nuclear transport, DNA replication and repair, mitosis, transcriptional regulation, and signal transduction. Alternatively spliced transcript variants encoding distinct proteins have been described. [provided by RefSeq, Feb 2014]

SUMO3: Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. Covalently attached to a number of proteins. Interacts with USP25 (via ts SIM domain); the interaction sumoylates USP25 and inhibits its ubiquitin hydrolyzing activity. Interacts with SAE2 and UBE2I. Expressed predominantly in liver. Belongs to the ubiquitin family. SUMO subfamily. Protein type: Ubiquitin-like modifier. Chromosomal Location of Human Ortholog: 21q22.3. Cellular Component: nucleoplasm; kinetochore; PML body; cytoplasm; nucleus. Molecular Function: protein binding; SUMO ligase activity. Biological Process: cellular protein metabolic process; protein sumoylation; post-translational protein modification

48-Strip-Wells

275 USD

96-Strip-Wells

420