ELISA/assay

Human Histamine H1 receptor, HRH1 ELISA Kit

MBS2602695

48-Strip-Wells

275 USD

ELISA Kit

Human Histamine H1 receptor, HRH1 ELISA Kit

Histamine H1 receptor, HRH1

histamine H1 receptor; Histamine H1 receptor; histamine H1 receptor; H1R; HH1R; histamine receptor, subclass H1; histamine receptor H1

HRH1

HRH1; HRH1; H1-R; hisH1; H1R; HH1R

HRH1_HUMAN

Human

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant HRH1 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human HRH1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

4504491

NP_000852.1

NM_000861.3

P35367

55,784 Da

Amine Ligand-binding Receptors Pathway (106363); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Histamine Receptors Pathway (106367)

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene was thought to be intronless until recently. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due to contraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in the central nervous system. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

H1R: In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system. Belongs to the G-protein coupled receptor 1 family. Protein type: Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1; Membrane protein, integral. Chromosomal Location of Human Ortholog: 3p25. Cellular Component: nucleoplasm; integral to plasma membrane; cytoplasm; plasma membrane. Molecular Function: G-protein coupled receptor activity; histamine receptor activity. Biological Process: G-protein coupled receptor protein signaling pathway; inositol phosphate-mediated signaling; eosinophil chemotaxis; positive regulation of vasoconstriction; regulation of synaptic plasticity; visual learning; positive regulation of inositol trisphosphate biosynthetic process; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of vascular permeability; inflammatory response; memory

48-Strip-Wells

275 USD

96-Strip-Wells

420