catalog number :
MBS2602694
products type :
ELISA Kit
products full name :
Human Peroxisomal trans-2-enoyl-CoA reductase, PECR ELISA Kit
products short name :
Peroxisomal trans-2-enoyl-CoA reductase, PECR
other names :
peroxisomal trans-2-enoyl-CoA reductase; Peroxisomal trans-2-enoyl-CoA reductase; peroxisomal trans-2-enoyl-CoA reductase; DCR-RP; pVI-ARL; 2,4-dienoyl-CoA reductase-related protein; putative short chain alcohol dehydrogenase; short chain dehydrogenase/reductase family 29C, member 1; peroxisomal trans-2-enoyl-CoA reductase; 2,4-dienoyl-CoA reductase-related protein; DCR-RP; HPDHase; pVI-ARL
products gene name :
PECR
other gene names :
PECR; PECR; TERP; DCRRP; PVIARL; HPDHASE; SDR29C1; HSA250303; PRO1004; TERP; DCR-RP
uniprot entry name :
PECR_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PECR concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human PECR monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_060911.2
ncbi gb acc num :
NM_018441.5
ncbi mol weight :
32,544 Da
ncbi pathways :
Biosynthesis Of Unsaturated Fatty Acids Pathway (83034); Biosynthesis Of Unsaturated Fatty Acids Pathway (429); Fatty Acid Biosynthesis Pathway (198873); Mitochondrial LC-Fatty Acid Beta-Oxidation Pathway (198817); Peroxisome Pathway (131226); Peroxisome Pathway (131126)
uniprot summary :
PECR: Participates in chain elongation of fatty acids. Has no 2,4-dienoyl-CoA reductase activity. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Oxidoreductase; EC 1.3.1.38; Lipid Metabolism - unsaturated fatty acid biosynthesis. Chromosomal Location of Human Ortholog: 2q35. Cellular Component: peroxisomal membrane; mitochondrion; intracellular membrane-bound organelle; peroxisome. Molecular Function: 2,4-dienoyl-CoA reductase (NADPH) activity; trans-2-enoyl-CoA reductase (NADPH) activity; receptor binding. Biological Process: phytol metabolic process; fatty acid biosynthetic process