ELISA/assay

Human Mitogen-activated protein kinase 10, MAPK10 ELISA Kit

MBS2602693

48-Strip-Wells

275 USD

ELISA Kit

Human Mitogen-activated protein kinase 10, MAPK10 ELISA Kit

Mitogen-activated protein kinase 10, MAPK10

mitogen-activated protein kinase 10 isoform 1; Mitogen-activated protein kinase 10; mitogen-activated protein kinase 10; MAPK 10; MAP kinase 10; MAP kinase p49 3F12; JNK3 alpha protein kinase; c-Jun N-terminal kinase 3; stress-activated protein kinase 1b; stress activated protein kinase beta; stress-activated protein kinase JNK3; mitogen-activated protein kinase 10; MAP kinase p49 3F12; Stress-activated protein kinase 1b; SAPK1b; Stress-activated protein kinase JNK3; c-Jun N-terminal kinase 3

MAPK10

MAPK10; MAPK10; JNK3; JNK3A; PRKM10; SAPK1b; p493F12; p54bSAPK; JNK3; JNK3A; PRKM10; SAPK1B; MAP kinase 10; MAPK 10; SAPK1b

MK10_HUMAN

Human

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant MAPK10 concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human MAPK10 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

4506081

NP_002744.1

NM_002753.3

P53779

52,585 Da

Activated TLR4 Signalling Pathway (106400); Activation Of The AP-1 Family Of Transcription Factors Pathway (160139); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Apoptosis Pathway (198797); Apoptosis Modulation By HSP70 Pathway (198877); CD40/CD40L Signaling Pathway (138061); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Colorectal Cancer Pathway (83106)

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This protein is a neuronal-specific form of c-Jun N-terminal kinases (JNKs). Through its phosphorylation and nuclear localization, this kinase plays regulatory roles in the signaling pathways during neuronal apoptosis. Beta-arrestin 2, a receptor-regulated MAP kinase scaffold protein, is found to interact with, and stimulate the phosphorylation of this kinase by MAP kinase kinase 4 (MKK4). Cyclin-dependent kianse 5 can phosphorylate, and inhibit the activity of this kinase, which may be important in preventing neuronal apoptosis. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

JNK3: a protein kinase of the MAPK family that is potently activated by a variety of environmental stress and pro-inflammatory cytokines. Brain-selective JNK isoform. A pro-apoptotic protein and potential tumor suppressor. Phosphorylates a number of transcription factors including c-Jun, ATF2 and ELK1. Required for stress-induced neuronal apoptosis and the pathogenesis of glutamate excitotoxicity. CDK5 can phosphorylate and inhibit the activity of this kinase, which may be important in preventing neuronal apoptosis. Binds to at least four scaffolding proteins, JIP-1, -2, -3 and -4. Interacts with HDAC9. Expression lost in brain tumors. May function in neuronal cell death from injury and neurodegeneration, for which inhibitors are being developed. Two alternatively spliced transcript variants have been reported. Protein type: Protein kinase, CMGC; Kinase, protein; EC 2.7.11.24; Protein kinase, Ser/Thr (non-receptor); CMGC group; MAPK family; MAPK/JNK subfamily; JNK subfamily. Chromosomal Location of Human Ortholog: 4q22.1-q23. Cellular Component: nucleoplasm; mitochondrion; cytoplasm; plasma membrane; cytosol. Molecular Function: MAP kinase kinase activity; protein binding; JUN kinase activity; ATP binding. Biological Process: response to light stimulus; activation of MAPK activity; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; rhythmic process; toll-like receptor 3 signaling pathway; signal transduction; regulation of circadian rhythm; protein amino acid phosphorylation; JUN phosphorylation; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription factor activity; toll-like receptor signaling pathway; innate immune response; JNK cascade; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway

48-Strip-Wells

275 USD

96-Strip-Wells

420