catalog number :
MBS2602690
products type :
ELISA Kit
products full name :
Human MKI67 FHA domain-interacting nucleolar phosphoprotein, MKI67IP ELISA Kit
products short name :
MKI67 FHA domain-interacting nucleolar phosphoprotein, MKI67IP
other names :
MKI67 FHA domain-interacting nucleolar phosphoprotein; MKI67 FHA domain-interacting nucleolar phosphoprotein; MKI67 FHA domain-interacting nucleolar phosphoprotein; hNIFK; nucleolar phosphoprotein Nopp34; MKI67 (FHA domain) interacting nucleolar phosphoprotein; nucleolar protein interacting with the FHA domain of pKi-67; nucleolar protein interacting with the FHA domain of MKI67; Nucleolar phosphoprotein Nopp34; Nucleolar protein interacting with the FHA domain of pKI-67; hNIFK
products gene name :
MKI67IP
other gene names :
NIFK; NIFK; Nopp34; MKI67IP; MKI67IP; NOPP34; hNIFK
uniprot entry name :
MK67I_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant MKI67IP concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human MKI67IP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_115766.3
ncbi gb acc num :
NM_032390.4
ncbi mol weight :
34,222 Da
ncbi summary :
This gene encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.[provided by RefSeq, Jan 2009]
uniprot summary :
NIFK: a nucleolar protein of unknown function that interacts with the fork-head associated (FHA) domain of the proliferation marker protein Ki-67. Protein type: RNA-binding; Nucleolus. Chromosomal Location of Human Ortholog: 2q14.3. Cellular Component: nucleoplasm; condensed nuclear chromosome; cytoplasm; nucleolus; nucleus. Molecular Function: protein binding; RNA binding; nucleotide binding. Biological Process: rRNA metabolic process; protein complex assembly; rRNA transcription