catalog number :
MBS2602679
products type :
ELISA Kit
products full name :
Mouse Plexin-A4, PLXNA4 ELISA Kit
products short name :
Plexin-A4, PLXNA4
other names :
plexin-A4; Plexin-A4; plexin-A4; plexin A4
products gene name :
PLXNA4
other gene names :
Plxna4; Plxna4; Plxa4; mKIAA1550; 9330117B14; Kiaa1550
uniprot entry name :
PLXA4_MOUSE
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PLXNA4 concentration. Assay Type: Sandwich. Detection Range: 100 ng/ml-1.56 ng/ml. Sensitivity: 0.5ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse PLXNA4 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_786926.2
ncbi gb acc num :
NM_175750.3
ncbi mol weight :
212,560 Da
ncbi pathways :
Axon Guidance Pathway (83262); Axon Guidance Pathway (476); Axon Guidance Pathway (970585); CRMPs In Sema3A Signaling Pathway (1000764); Developmental Biology Pathway (970813); Other Semaphorin Interactions Pathway (1000765); SEMA3A-Plexin Repulsion Signaling By Inhibiting Integrin Adhesion Pathway (1000762); Sema3A PAK Dependent Axon Repulsion Pathway (1000763); Semaphorin Interactions Pathway (1000758)
uniprot summary :
PLXNA4: Coreceptor for SEMA3A. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance in the developing nervous system. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm. Interacts with NRP1 and NRP2. Belongs to the plexin family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Cellular Component: membrane; plasma membrane; integral to membrane; intracellular. Molecular Function: protein binding; semaphorin receptor activity; receptor activity. Biological Process: facial nerve structural organization; nervous system development; axon guidance; trigeminal nerve structural organization; multicellular organismal development; glossopharyngeal nerve morphogenesis; facial nerve morphogenesis; motor axon guidance; regulation of negative chemotaxis; signal transduction; trigeminal nerve morphogenesis; sympathetic nervous system development; anterior commissure morphogenesis; branchiomotor neuron axon guidance; cranial nerve morphogenesis; regulation of axon extension involved in axon guidance; postganglionic parasympathetic nervous system development; vagus nerve morphogenesis; neurite morphogenesis; chemorepulsion of branchiomotor axon