catalog number :
MBS2602677
products type :
ELISA Kit
products full name :
Mouse N-chimaerin, CHN1 ELISA Kit
products short name :
N-chimaerin, CHN1
other names :
N-chimaerin isoform 3; N-chimaerin; N-chimaerin; NC; N-chimerin; A-chimaerin; alpha-chimerin; alpha3-chimerin; alpha1 chimaerin; alpha2 chimaerin; chimerin (chimaerin) 1; rho GTPase-activating protein 2; chimerin 1; A-chimaerin; Alpha-chimerin; N-chimerin; NC; Rho GTPase-activating protein 2
products gene name :
CHN1
other gene names :
Chn1; Chn1; ARHGAP2; AI413815; 0610007I19Rik; 0710001E19Rik; 1700112L09Rik; 2900046J01Rik; Arhgap2; NC
uniprot entry name :
CHIN_MOUSE
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant CHN1 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse CHN1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_001106717.2
ncbi gb acc num :
NM_001113246.2
ncbi mol weight :
53,193 Da
ncbi pathways :
Rho GTPase Cycle Pathway (1000930); Signal Transduction Pathway (970540); Signaling By Rho GTPases Pathway (1000929)
uniprot summary :
ARHGAP2: GTPase-activating protein for p21-rac and a phorbol ester receptor. Involved in the assembly of neuronal locomotor circuits as a direct effector of EPHA4 in axon guidance. Interacts with EPHA4; effector of EPHA4 in axon guidance linking EPHA4 activation to RAC1 regulation. In neurons in brain regions that are involved in learning and memory processes. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Adaptor/scaffold; GAPs; GAPs, Rac/Rho. Cellular Component: intracellular. Molecular Function: protein binding; ephrin receptor binding; metal ion binding; GTPase activator activity. Biological Process: nervous system development; regulation of GTPase activity; ephrin receptor signaling pathway; motor axon guidance; regulation of axonogenesis; signal transduction