catalog number :
MBS2602676
products type :
ELISA Kit
products full name :
Rat Carnitine palmitoyltransferase I, CPT1 ELISA Kit
products short name :
Carnitine palmitoyltransferase I, CPT1
other names :
carnitine O-palmitoyltransferase 1, liver isoform; Carnitine O-palmitoyltransferase 1, liver isoform; carnitine O-palmitoyltransferase 1, liver isoform; CPT I; CPT1-L; CPTI-L; carnitine palmitoyltransferase 1, liver; carnitine O-palmitoyltransferase I, liver isoform; Carnitine palmitoyltransferase 1 alpha, liver isoform; carnitine palmitoyltransferase 1a, liver; Carnitine O-palmitoyltransferase I, liver isoform; CPT I; CPTI-L; Carnitine palmitoyltransferase 1A
products gene name :
CPT1
other gene names :
Cpt1a; Cpt1a; CPT-Ia; Cpt-1; Cpt1; CPT1-L; CPT I; CPTI-L
uniprot entry name :
CPT1A_RAT
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Rat serum, plasma or cell culture supernatant and organizations in the natural and recombinant CPT1 concentration. Assay Type: Sandwich. Detection Range: 200 ng/ml-3.12 ng/ml. Sensitivity: Up to 0.6 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat CPT1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_113747.2
ncbi gb acc num :
NM_031559.2
ncbi mol weight :
88,125 Da
ncbi pathways :
AMPK Signaling Pathway (989832); AMPK Signaling Pathway (992181); Adipocytokine Signaling Pathway (83485); Adipocytokine Signaling Pathway (505); Beta Oxidation Meta Pathway (198526); Circadian Clock Pathway (1010370); Fatty Acid Beta Oxidation Pathway (219782); Fatty Acid Beta Oxidation 1 Pathway (198531); Fatty Acid Degradation Pathway (83328); Fatty Acid Degradation Pathway (296)
ncbi summary :
enzyme that catalyses the transfer of long chain fatty acids to carnitine for translocation across the mitochondrial inner membrane [RGD, Feb 2006]
uniprot summary :
CPT1A: Belongs to the carnitine/choline acetyltransferase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 2.3.1.21; Membrane protein, multi-pass; Transferase; Membrane protein, integral; Lipid Metabolism - fatty acid; Mitochondrial. Cellular Component: endoplasmic reticulum membrane; mitochondrial outer membrane; membrane; intracellular membrane-bound organelle; mitochondrion; mitochondrial inner membrane; integral to membrane; integral to mitochondrial outer membrane. Molecular Function: identical protein binding; protein binding; carnitine O-palmitoyltransferase activity. Biological Process: response to drug; eating behavior; glucose metabolic process; long-chain fatty acid metabolic process; response to organic cyclic substance; positive regulation of fatty acid beta-oxidation; fatty acid beta-oxidation; triacylglycerol metabolic process; epithelial cell differentiation; transport; regulation of fatty acid oxidation; regulation of fatty acid beta-oxidation; carnitine metabolic process; regulation of insulin secretion; protein homooligomerization