ELISA/assay

Human Lymphoid enhancer-binding factor 1, LEF1 ELISA Kit

MBS2602672

48-Strip-Wells

275 USD

ELISA Kit

Human Lymphoid enhancer-binding factor 1, LEF1 ELISA Kit

Lymphoid enhancer-binding factor 1, LEF1

lymphoid enhancer-binding factor 1 isoform 2; Lymphoid enhancer-binding factor 1; lymphoid enhancer-binding factor 1; TCF1-alpha; T cell-specific transcription factor 1-alpha; lymphoid enhancer-binding factor 1; T cell-specific transcription factor 1-alpha; TCF1-alpha

LEF1

LEF1; LEF1; LEF-1; TCF10; TCF7L3; TCF1ALPHA; LEF-1; TCF1-alpha

LEF1_HUMAN

Human

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant LEF1 concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human LEF1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

195222732

NP_001124185.1

NM_001130713.2

Q9UJU2

44,201 Da

Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117293); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); C-MYB Transcription Factor Network Pathway (138073); Colorectal Cancer Pathway (83106)

This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

LEF-1: a transcription factor of the TCF/LEF family. Participates in the Wnt signaling pathway and regulates T-cell receptor alpha-C enhancer function. Activates transcription of target genes in the presence of CTNNB1 and EP300. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1. Deletions in LEF1 have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Four human isoforms produced by alternative promoter usage and alternative splicing have been described. Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 4q23-q25. Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus. Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; estrogen receptor activity; histone binding; caspase inhibitor activity; beta-catenin binding; transcription factor binding; protein binding; DNA binding; sequence-specific DNA binding; gamma-catenin binding; estrogen receptor binding; chromatin binding; DNA bending activity; transcription factor activity. Biological Process: radial glial cell differentiation in the forebrain; hypothalamus development; positive regulation of transcription, DNA-dependent; T-helper 1 cell differentiation; Wnt receptor signaling pathway through beta-catenin; forebrain neuroblast division; forebrain neuron differentiation; BMP signaling pathway; negative regulation of interleukin-13 production; neural crest cell migration; regulation of striated muscle development; embryonic limb morphogenesis; somitogenesis; dentate gyrus development; sensory perception of taste; negative regulation of striated muscle development; positive regulation of cell growth; patterning of blood vessels; negative regulation of interleukin-5 production; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; transcription from RNA polymerase II promoter; B cell proliferation; negative regulation of interleukin-4 production; tongue development; paraxial mesoderm formation; positive regulation of granulocyte differentiation; alpha-beta T cell differentiation; palate development; negative regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; anatomical structure regression; regulation of cell-cell adhesion; mammary gland development; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; muscle fiber development; Wnt receptor signaling pathway; neutrophil differentiation; negative regulation of DNA binding; odontogenesis of dentine-containing teeth; formation of radial glial scaffolds; osteoblast differentiation; T cell receptor V(D)J recombination; epithelial to mesenchymal transition; sprouting angiogenesis; negative regulation of cell-cell adhesion; eye pigmentation; positive regulation of cell migration

48-Strip-Wells

275 USD

96-Strip-Wells

420