catalog number :
MBS2602671
products type :
ELISA Kit
products full name :
Human Dicer-1 ELISA Kit
products short name :
Dicer-1
other names :
Dicer-1; Endoribonuclease Dcr-1; CG4792 gene product from transcript CG4792-RA; CG4792-PA; Dcr-1-PA; Dicer-1; dicer; dicer1; Dicer-1
other gene names :
Dcr-1; Dcr-1; cg4792; CG4792; Dcr; dcr 1; dcr-1; DCR-1; dcr1; Dcr1; DCR1; dicer; Dicer; DICER; dicer-1; dicer1; Dicer1; DmelCG4792; CG4792; Protein dicer-1
uniprot entry name :
DCR1_DROME
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant Dicer-1 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human Dicer-1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_524453.1
ncbi gb acc num :
NM_079729.3
ncbi mol weight :
255,330 Da
ncbi pathways :
Gene Expression Pathway (1006408); MicroRNA (miRNA) Biogenesis Pathway (1006469); Regulatory RNA Pathways (1006468); Small Interfering RNA (siRNA) Biogenesis Pathway (1006470); MRNA Processing Pathway (198267)
uniprot summary :
Function: Essential for RNA interference (RNAi); double-stranded RNA (dsRNA) induces potent and specific gene silencing. RNAi is mediated by the RNA-induced silencing complex (RISC), a sequence-specific, multicomponent nuclease that destroys or silences messenger RNAs homologous to the silencing trigger. May carry out the initiation step of RNAi by cleaving dsRNA to produce 22 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs. During embryogenesis, involved in germline fate determination.3 PublicationsManual assertion based on experiment in:Ref.4. Cofactor: Magnesium or manganese