catalog number :
MBS2602667
products type :
ELISA Kit
products full name :
Human Guanine nucleotide-binding protein G, GNG8 ELISA Kit
products short name :
Guanine nucleotide-binding protein G, GNG8
other names :
guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-8; Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-8; guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-8; guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-8; gamma-9; guanine nucleotide binding protein (G protein), gamma 8; Gamma-9
products gene name :
GNG8
other gene names :
GNG8; GNG8; GNG9; GNGT9
uniprot entry name :
GBG8_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant GNG8 concentration. Assay Type: Sandwich. Detection Range: 100 ng/ml-1.56 ng/ml. Sensitivity: 0.5 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human GNG8 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_150283.1
ncbi gb acc num :
NM_033258.1
ncbi mol weight :
7,841 Da
ncbi pathways :
ADP Signalling Through P2Y Purinoceptor 1 Pathway (161061); ADP Signalling Through P2Y Purinoceptor 12 Pathway (161060); Activation Of G Protein Gated Potassium Channels Pathway (366226); Activation Of GABAB Receptors Pathway (187181); Activation Of Kainate Receptors Upon Glutamate Binding Pathway (161043); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Aquaporin-mediated Transport Pathway (187187); Calcium Regulation In The Cardiac Cell Pathway (198906); Chemokine Signaling Pathway (99051)
uniprot summary :
G-gamma 8: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction. Belongs to the G protein gamma family. Protein type: G protein, heterotrimeric gamma. Chromosomal Location of Human Ortholog: 19q13.32. Cellular Component: plasma membrane; heterotrimeric G-protein complex. Molecular Function: GTPase activity; protein binding; signal transducer activity. Biological Process: synaptic transmission; nervous system development; G-protein coupled receptor protein signaling pathway; energy reserve metabolic process