catalog number :
MBS2602666
products type :
ELISA Kit
products full name :
Bovine Monofunctional C1-tetrahydrofolate synthase, mitochondrial, MTHFD1L ELISA Kit
products short name :
Monofunctional C1-tetrahydrofolate synthase, mitochondrial, MTHFD1L
other names :
monofunctional C1-tetrahydrofolate synthase, mitochondrial; Monofunctional C1-tetrahydrofolate synthase, mitochondrial; monofunctional C1-tetrahydrofolate synthase, mitochondrial; formyltetrahydrofolate synthetase; methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like<; Formyltetrahydrofolate synthetase
products gene name :
MTHFD1L
other gene names :
MTHFD1L; MTHFD1L
uniprot entry name :
C1TM_BOVIN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Bovine serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant MTHFD1L concentration. Assay Type: Sandwich. Detection Range: 100 ng/ml-1.56 ng/ml. Sensitivity: The minimum detectable Bovine MTHFD1L up to 0.5 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Bovine MTHFD1L monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_001069486.1
ncbi gb acc num :
NM_001076018.2
ncbi mol weight :
105,227 Da
ncbi pathways :
C1-unit Interconversion, Eukaryotes Pathway (391031); C1-unit Interconversion, Eukaryotes Pathway (468334); One Carbon Pool By Folate Pathway (84249); One Carbon Pool By Folate Pathway (393); Folate Polyglutamylation I Pathway (123010); Formaldehyde Oxidation V (tetrahydrofolate Pathway) (123109); FormylTHF Biosynthesis II Mammals Pathway (123014); Glycine Degradation I Pathway (123086); Histidine Degradation III Mammals Pathway (123089); Tetrahydrofolate Biosynthesis I Mammals Pathway (123013)
uniprot summary :
Function: May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2 UniRule annotationHAMAP-Rule:MF_01543. Catalytic activity: ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotationHAMAP-Rule:MF_01543