ELISA/assay

Human Obscurin, OBSCN ELISA Kit

MBS2602663

48-Strip-Wells

275 USD

ELISA Kit

Human Obscurin, OBSCN ELISA Kit

Obscurin, OBSCN

obscurin isoform b; Obscurin; obscurin; obscurin-MLCK; obscurin, myosin light chain kinase; obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF; Obscurin-RhoGEF; Obscurin-myosin light chain kinase; Obscurin-MLCK

OBSCN

OBSCN; OBSCN; UNC89; ARHGEF30; KIAA1556; KIAA1639; Obscurin-MLCK

OBSCN_HUMAN

Human

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant OBSCN concentration. Assay Type: Sandwich. Detection Range: 100 ng/ml-1.56 ng/ml. Sensitivity: 0.5 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human OBSCN monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

403501446

NP_001092093.2

NM_001098623.2

Q5VST9

868,484 Da

Cell Death Signalling Via NRAGE, NRIF And NADE Pathway (106443); G Alpha (12/13) Signalling Events Pathway (106041); GPCR Downstream Signaling Pathway (119548); NRAGE Signals Death Through JNK Pathway (106444); Regulation Of RhoA Activity Pathway (138070); Rho GTPase Cycle Pathway (106503); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356); Signaling By Rho GTPases Pathway (106502); Signalling By NGF Pathway (106439)

The obscurin gene spans more than 150 kb, contains over 80 exons and encodes a protein of approximately 720 kDa. The encoded protein contains 68 Ig domains, 2 fibronectin domains, 1 calcium/calmodulin-binding domain, 1 RhoGEF domain with an associated PH domain, and 2 serine-threonine kinase domains. This protein belongs to the family of giant sacromeric signaling proteins that includes titin and nebulin, and may have a role in the organization of myofibrils during assembly and may mediate interactions between the sarcoplasmic reticulum and myofibrils. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

OBSCN: a Ca(2+)/calmodulin-dependent protein kinase of the Trio family. A giant protein with one Rho GEF domain, one PH domain and two kinase domains. Expressed in skeletal and cardiac muscle, concentrating around Z-disks and M-lines of striated muscle. Binds ankyrin 1 interacts with sarcomeric myosin. May play a critical role in its ability to assemble into A bands in striated muscle. Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); CAMK group; Trio family. Chromosomal Location of Human Ortholog: 1q42.13. Cellular Component: myofibril; M band; cytosol; Z disc. Molecular Function: calmodulin binding; protein serine/threonine kinase activity; Rho guanyl-nucleotide exchange factor activity; protein binding; structural constituent of muscle; metal ion binding; titin binding; ankyrin binding; ATP binding. Biological Process: regulation of small GTPase mediated signal transduction; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; small GTPase mediated signal transduction; multicellular organismal development; sarcomere organization; protein amino acid phosphorylation

48-Strip-Wells

275 USD

96-Strip-Wells

420