ELISA/assay

Human Protein atonal homolog 1, ATOH1 ELISA Kit

MBS2602662

48-Strip-Wells

275 USD

ELISA Kit

Human Protein atonal homolog 1, ATOH1 ELISA Kit

Protein atonal homolog 1, ATOH1

protein atonal homolog 1; Protein atonal homolog 1; protein atonal homolog 1; helix-loop-helix protein hATH-1; class A basic helix-loop-helix protein 14; atonal homolog 1 (Drosophila); Class A basic helix-loop-helix protein 14; bHLHa14; Helix-loop-helix protein hATH-1; hATH1

ATOH1

ATOH1; ATOH1; ATH1; HATH1; MATH-1; bHLHa14; ATH1; BHLHA14; bHLHa14; hATH1

ATOH1_HUMAN

Human

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant ATOH1 concentration. Assay Type: Sandwich. Detection Range: 100 ng/ml-1.56 ng/ml. Sensitivity: 0.5 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human ATOH1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

4885075

NP_005163.1

NM_005172.1

Q92858

38,160 Da

This protein belongs to the basic helix-loop-helix (BHLH) family of transcription factors. It activates E-box dependent transcription along with E47. [provided by RefSeq, Jul 2008]

ATOH1: a conserved transcription factor that regulates secretory cell differentiation in colonic epithelium. Activates E box-dependent transcription in collaboration with TCF3/E47, but the activity is completely antagonized by the negative regulator of neurogenesis HES1. May play a role in the differentiation of subsets of neural cells by activating E box- dependent transcription . Efficient DNA binding requires dimerization with another bHLH protein. Loss of ATOH1 expression in the colon of mice initiates colon cancer. ATOH1 has been silenced in most human colon cancers, and reactivation of this gene in cultured human colon cancer cells is anti-proliferative and induces apoptosis. Protein type: Transcription factor; Cell development/differentiation; DNA-binding. Chromosomal Location of Human Ortholog: 4q22. Cellular Component: nucleus. Molecular Function: protein dimerization activity; chromatin DNA binding; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; axon guidance; inner ear morphogenesis; central nervous system development; auditory receptor cell fate determination; neuron migration; positive regulation of transcription from RNA polymerase II promoter; auditory receptor cell fate specification; positive regulation of auditory receptor cell differentiation; cerebral cortex development; positive regulation of neuron differentiation; negative regulation of apoptosis

48-Strip-Wells

275 USD

96-Strip-Wells

420