ELISA/assay

Human Succinate dehydrogenase cytochrome b560 subunit, mitochondrial, SDHC ELISA Kit

MBS2602659

48-Strip-Wells

275 USD

ELISA Kit

Human Succinate dehydrogenase cytochrome b560 subunit, mitochondrial, SDHC ELISA Kit

Succinate dehydrogenase cytochrome b560 subunit, mitochondrial, SDHC

succinate dehydrogenase cytochrome b560 subunit, mitochondrial isoform 2; Succinate dehydrogenase cytochrome b560 subunit, mitochondrial; succinate dehydrogenase cytochrome b560 subunit, mitochondrial; integral membrane protein CII-3b; succinate dehydrgenase cytochrome b; cytochrome B large subunit of complex II; succinate dehydrogenase 3, integral membrane subunit; succinate-ubiquinone oxidoreductase cytochrome B large subunit; succinate dehydrogenase complex, subunit C, integral membrane protein, 15kD; succinate dehydrogenase complex, subunit C, integral membrane protein, 15kDa; Integral membrane protein CII-3; QPs-1; QPs1; Succinate dehydrogenase complex subunit C; Succinate-ubiquinone oxidoreductase cytochrome B large subunit; CYBL

SDHC

SDHC; SDHC; CYBL; PGL3; QPS1; SDH3; CYB560; CYB560; SDH3; QPs1; CYBL

C560_HUMAN

Human

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant SDHC concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human SDHC monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

78711820

NP_001030588.1

NM_001035511.1

Q99643

18,610 Da

Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Citrate Cycle (TCA Cycle) Pathway (82927); Citrate Cycle (TCA Cycle) Pathway (288); Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate = Oxaloacetate Pathway (413348); Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate = Oxaloacetate Pathway (468204); Citric Acid Cycle (TCA Cycle) Pathway (105919); Electron Transport Chain Pathway (198860); Huntington's Disease Pathway (83100); Huntington's Disease Pathway (512)

This gene encodes one of four nuclear-encoded subunits that comprise succinate dehydrogenase, also known as mitochondrial complex II, a key enzyme complex of the tricarboxylic acid cycle and aerobic respiratory chains of mitochondria. The encoded protein is one of two integral membrane proteins that anchor other subunits of the complex, which form the catalytic core, to the inner mitochondrial membrane. There are several related pseudogenes for this gene on different chromosomes. Mutations in this gene have been associated with paragangliomas. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2013]

SDHC: Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Defects in SDHC are the cause of paragangliomas type 3 (PGL3). A neural crest tumor usually derived from the chromoreceptor tissue of a paraganglion. Paragangliomas are most commonly located in the head and neck region, specifically at the carotid bifurcation, the jugular foramen, the vagal nerve, and in the middle ear. Defects in SDHC are a cause of paraganglioma and gastric stromal sarcoma (PGGSS); also known as Carney- Stratakis syndrome. Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant manner, with incomplete penetrance. Belongs to the cytochrome b560 family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Energy Metabolism - oxidative phosphorylation; Membrane protein, multi-pass; Mitochondrial; Carbohydrate Metabolism - citrate (TCA) cycle; Membrane protein, integral. Chromosomal Location of Human Ortholog: 1q23.3. Cellular Component: mitochondrial respiratory chain complex II; mitochondrion; mitochondrial inner membrane; integral to membrane. Molecular Function: electron carrier activity; metal ion binding; heme binding; succinate dehydrogenase activity. Biological Process: cellular metabolic process; tricarboxylic acid cycle; aerobic respiration. Disease: Gastrointestinal Stromal Tumor; Paraganglioma And Gastric Stromal Sarcoma; Paragangliomas 3

48-Strip-Wells

275 USD

96-Strip-Wells

420