Human Transcription intermediary factor 1-beta, TRIM28/KAP1/RNF96/TIF1B ELISA Kit | MyBioSource MBS2602655 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Transcription intermediary factor 1-beta, TRIM28/KAP1/RNF96/TIF1B ELISA Kit

catalog :

MBS2602655

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2602655

products type :

ELISA Kit

products full name :

Human Transcription intermediary factor 1-beta, TRIM28/KAP1/RNF96/TIF1B ELISA Kit

products short name :

Transcription intermediary factor 1-beta, TRIM28/KAP1/RNF96/TIF1B

other names :

transcription intermediary factor 1-beta; Transcription intermediary factor 1-beta; transcription intermediary factor 1-beta; KAP-1; KRIP-1; TIF1-beta; RING finger protein 96; KRAB-associated protein 1; nuclear corepressor KAP-1; KRAB-interacting protein 1; E3 SUMO-protein ligase TRIM28; tripartite motif-containing 28; tripartite motif-containing protein 28; transcriptional intermediary factor 1-beta; protein phosphatase 1, regulatory subunit 157; tripartite motif containing 28; E3 SUMO-protein ligase TRIM28 (EC:6.3.2.-); KRAB-associated protein 1; KAP-1; KRAB-interacting protein 1; KRIP-1; Nuclear corepressor KAP-1; RING finger protein 96; Tripartite motif-containing protein 28

products gene name :

TRIM28/KAP1/RNF96/TIF1B

other gene names :

TRIM28; TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157; KAP1; RNF96; TIF1B; TIF1-beta; KAP-1; KRIP-1

uniprot entry name :

TIF1B_HUMAN

reactivity :

Human

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant TRIM28 concentration. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: Up to 0.06 ng/ml. Assay Type: Sandwich

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human TRIM28 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

5032179

ncbi acc num :

NP_005753.1

ncbi gb acc num :

NM_005762.2

uniprot acc num :

Q13263

ncbi mol weight :

88,550 Da

ncbi pathways :

C-MYB Transcription Factor Network Pathway (138073); Corticotropin-releasing Hormone Pathway (920957); E2F Transcription Factor Network Pathway (137934); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); P53 Pathway (138067)

ncbi summary :

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]

uniprot summary :

TIF1B: an atypical protein kinase that functions as a transcriptional coactivator. Mediates transcriptional regulation by interaction with the Kruppel-associated box (KRAB) repression domain found in many transcription factors. Binds to the negative regulatory domain of c-Myb and negatively regulates the c-Myb-dependent trans-activation. Localizes to the nucleus and is thought to associate with specific chromatin regions. Phosphorylated in an ATM-dependent manner following double-strand breaks. Its phosphorylation in response to DNA damage leads to chromatin relaxation. A member of the tripartite motif family. The tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Protein type: Kinase, protein; Ubiquitin conjugating system; Ubiquitin ligase; Nuclear receptor co-regulator; Protein kinase, atypical; Ligase; EC 6.3.2.-; Transcription, coactivator/corepressor; ATYPICAL group; TIF1 family. Chromosomal Location of Human Ortholog: 19q13.4. Cellular Component: nucleoplasm; nuclear heterochromatin; nucleus. Molecular Function: protein binding; DNA binding; zinc ion binding; sequence-specific DNA binding; ubiquitin protein ligase binding; transcription coactivator activity; ubiquitin-protein ligase activity; transcription corepressor activity; transcription factor activity; protein kinase activity; ligase activity. Biological Process: transcription initiation from RNA polymerase II promoter; convergent extension involved in axis elongation; positive regulation of DNA repair; positive regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter; DNA repair; protein oligomerization; protein sumoylation; positive regulation of transcription factor import into nucleus; innate immune response; epithelial to mesenchymal transition; gene expression; negative regulation of transcription, DNA-dependent; DNA methylation during embryonic development; embryo implantation

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

420

more info or order :

MyBioSource product webpage