Human Secretory phospholipase A2 receptor, PLA2R1 antibody ELISA Kit | MyBioSource MBS2602654 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Secretory phospholipase A2 receptor, PLA2R1 antibody ELISA Kit

catalog :

MBS2602654

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2602654

products type :

ELISA Kit

products full name :

Human Secretory phospholipase A2 receptor, PLA2R1 antibody ELISA Kit

products short name :

Secretory phospholipase A2 receptor, PLA2R1 antibody

other names :

secretory phospholipase A2 receptor isoform 3; Secretory phospholipase A2 receptor; secretory phospholipase A2 receptor; M-type receptor; phospholipase A2 receptor 1, 180kD; C-type lectin domain family 13 member C; 180 kDa secretory phospholipase A2 receptor; phospholipase A2 receptor 1, 180kDa; 180 kDa secretory phospholipase A2 receptor; C-type lectin domain family 13 member C; M-type receptorCleaved into the following chain:Soluble secretory phospholipase A2 receptor; Soluble PLA2-R; Soluble PLA2R

products gene name :

PLA2R1

other gene names :

PLA2R1; PLA2R1; PLA2R; PLA2-R; PLA2IR; CLEC13C; PLA2G1R; CLEC13C; PLA2-R; PLA2R; Soluble PLA2-R; Soluble PLA2R

uniprot entry name :

PLA2R_HUMAN

reactivity :

Human

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PLA2R1 Ab concentration. Assay Type: Sandwich. Detection Range: 100 U/ml-1.56 U/ml. Sensitivity: 0.5 U/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human PLA2R1 Ab monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

307548857

ncbi acc num :

NP_001182570.1

ncbi gb acc num :

NM_001195641.1

uniprot acc num :

Q13018

ncbi mol weight :

152,805 Da

ncbi pathways :

Phagosome Pathway (153910); Phagosome Pathway (153859); Tuberculosis Pathway (213780); Tuberculosis Pathway (213743)

ncbi summary :

This gene represents a phospholipase A2 receptor. The encoded protein likely exists as both a transmembrane form and a soluble form. The transmembrane receptor may play a role in clearance of phospholipase A2, thereby inhibiting its action. Polymorphisms at this locus have been associated with susceptibility to idiopathic membranous nephropathy. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Sep 2010]

uniprot summary :

PLA2R1: Receptor for secretory phospholipase A2 (sPLA2). Acts as a receptor for phosholipase sPLA2-IB/PLA2G1B but not sPLA2- IIA/PLA2G2A. Also able to bind to snake PA2-like toxins. Although its precise function remains unclear, binding of sPLA2 to its receptor participates in both positive and negative regulation of sPLA2 functions as well as clearance of sPLA2. Binding of sPLA2- IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogen-activated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipid mediators, selective release of arachidonic acid in bone marrow- derived mast cells. In neutrophils, binding of sPLA2-IB/PLA2G1B can activate p38 MAPK to stimulate elastase release and cell adhesion. May be involved in responses in proinflammatory cytokine productions during endotoxic shock. Also has endocytic properties and rapidly internalizes sPLA2 ligands, which is particularly important for the clearance of extracellular sPLA2s to protect their potent enzymatic activities. The soluble secretory phospholipase A2 receptor form is circulating and acts as a negative regulator of sPLA2 functions by blocking the biological functions of sPLA2-IB/PLA2G1B. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 2q23-q24. Cellular Component: cell surface; integral to plasma membrane; integral to membrane; plasma membrane; extracellular region; receptor complex. Molecular Function: phospholipase binding; receptor activity; carbohydrate binding. Biological Process: receptor-mediated endocytosis; cytokine production; endocytosis; positive regulation of DNA damage response, signal transduction by p53 class mediator

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

420

more info or order :

MyBioSource product webpage