ELISA/assay

Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1, SMARCC1 ELISA Kit

MBS2602643

48-Strip-Wells

275 USD

ELISA Kit

Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1, SMARCC1 ELISA Kit

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1, SMARCC1

SWI/SNF complex subunit SMARCC1; SWI/SNF complex subunit SMARCC1; SWI/SNF complex subunit SMARCC1; BRG1-associated factor 155; SWI/SNF complex 155 kDa subunit; chromatin remodeling complex BAF155 subunit; mammalian chromatin remodeling complex BRG1-associated factor 155; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1; BRG1-associated factor 155; BAF155; SWI/SNF complex 155 kDa subunit; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1

SMARCC1

SMARCC1; SMARCC1; Rsc8; SRG3; SWI3; BAF155; CRACC1; BAF155; BAF155

SMRC1_HUMAN

Human

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant SMARCC1 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human SMARCC1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

188536047

NP_003065.3

NM_003074.3

Q92922

122,867 Da

Chromatin Modifying Enzymes Pathway (941403); Chromatin Organization Pathway (941402); Glucocorticoid Receptor Regulatory Network Pathway (138014); Prostate Cancer Pathway (755440); RMTs Methylate Histone Arginines Pathway (1016172); Regulation Of Androgen Receptor Activity Pathway (138027); TNF-alpha/NF-kB Signaling Pathway (198884)

The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors. [provided by RefSeq, Jul 2008]

SMARCC1: a core component of the BAF (SWI/SNF) complex. This family of complexes remodels chromatin structures, enabling transcription factors to gain access to DNA. They play important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling is due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Belongs to the neural progenitors- specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain BAF53A and PHF10, are exchanged for homologous alternative BAF53B and BAF45B or BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand- bound VDR-mediated transrepression of the CYP27B1 gene. Component of 6 multiprotein chromatin-remodeling complexes: Swi/Snf-A (BAF), Swi/Snf-B (PBAF), Brm, Brg1(I), WINAC and Brg1(II). Each of the five complexes contains a catalytic subunit (either SMARCA4 or SMARCA2), and at least SMARCE1, BAF53A or BAF53B, SMARCC2 and SMARCB1. Other subunits specific to each of the complexes may also be present. Component of the BAF (hSWI/SNF) complex, which includes at least actin (ACTB), ARID1A, ARID1B, SMARCA2, SMARCA4, BAF53A, BAF53B, SMARCE1 SMARCC1, SMARCC2, SMARCB1, and one or more of SMARCD1, SMARCD2, or SMARCD3. In muscle cells, the BAF complex also contains DPF3. May also interact with the SIN3A histone deacetylase transcription repressor complex in conjunction with SMARCA2 and SMARCA4. The minimal complex composed of SMARCC1 and SMARCA4 seems to be able to associate with cyclin such as CCNE1 or transcription factors such as KLF1 or GATA1. Expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas. Belongs to the SMARCC family. Protein type: DNA-binding. Chromosomal Location of Human Ortholog: 3p21.31. Cellular Component: nucleoplasm; XY body; SWI/SNF complex; protein complex; nuclear chromatin. Molecular Function: protein binding; nucleosomal DNA binding; transcription coactivator activity; protein N-terminus binding. Biological Process: regulation of transcription from RNA polymerase II promoter; chromatin remodeling; nervous system development; establishment and/or maintenance of chromatin architecture; organ morphogenesis; transcription, DNA-dependent; nucleosome disassembly; positive regulation of transcription, DNA-dependent; ATP-dependent chromatin remodeling; insulin receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter

48-Strip-Wells

275 USD

96-Strip-Wells

420