catalog number :
MBS2602641
products type :
ELISA Kit
products full name :
Human Pulmonary surfactant-associated protein A (SFTPA1) ELISA Kit
products short name :
Pulmonary surfactant-associated protein A (SFTPA1)
other names :
SP-A; Pulmonary surfactant-associated protein A; pulmonary surfactant-associated protein A; PSAP; PSP-A; surfactant pulmonary associated protein A1; surfactant associated protein A1
products gene name :
SFTPA1
other gene names :
Sftpa1; SFTPA1; SP-A; Sftp1; Sftp-1; SFTPA; PSAP; PSP-A; SP-A
uniprot entry name :
SFTPA_BOVIN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant SFTPA concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human SFTPA monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_001071306.2
ncbi mol weight :
26,157 Da
ncbi pathways :
Pertussis Pathway (218112); Pertussis Pathway (218099); Phagosome Pathway (153914); Phagosome Pathway (153859)
uniprot summary :
SFTPA2: In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Defects in SFTPA2 are a cause of pulmonary fibrosis idiopathic (IPF). Pulmonary fibrosis is a lung disease characterized by shortness of breath, radiographically evident diffuse pulmonary infiltrates, and varying degrees of inflammation and fibrosis on biopsy. It results in acute lung injury with subsequent scarring and endstage lung disease. Belongs to the SFTPA family. Protein type: Secreted; Secreted, signal peptide. Cellular Component: multivesicular body; extracellular space; rough endoplasmic reticulum; cytoplasmic vesicle. Biological Process: positive regulation of phagocytosis