ELISA/assay

Mouse Mitogen-activated protein kinase 8, MAPK8 ELISA Kit

MBS2602636

48-Strip-Wells

290 USD

ELISA Kit

Mouse Mitogen-activated protein kinase 8, MAPK8 ELISA Kit

Mitogen-activated protein kinase 8, MAPK8

mitogen-activated protein kinase 8; Mitogen-activated protein kinase 8; mitogen-activated protein kinase 8; MAPK 8; MAP kinase 8; JNK1 beta1 protein kinase; c-Jun N-terminal kinase 1; mitogen activated protein kinase 8; protein kinase mitogen-activated 8; stress-activated protein kinase JNK1; mitogen-activated protein kinase 8; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1

MAPK8

Mapk8; Mapk8; JNK; JNK1; Prkm8; SAPK1; AI849689; Jnk1; Prkm8; MAP kinase 8; MAPK 8

MK08_MOUSE

Mouse

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant MAPK8 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse MAPK8 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

7710060

NP_057909.1

NM_016700.4

Q91Y86

44,229 Da

Activated TLR4 Signalling Pathway (575140); Activation Of BH3-only Proteins Pathway (574596); Activation Of BMF And Translocation To Mitochondria Pathway (574600); Activation Of The AP-1 Family Of Transcription Factors Pathway (575127); Adipocytokine Signaling Pathway (83290); Adipocytokine Signaling Pathway (505); Apoptosis Pathway (574586); B Cell Receptor Signaling Pathway (198285); Cell Death Signalling Via NRAGE, NRIF And NADE Pathway (575365); Chagas Disease (American Trypanosomiasis) Pathway (147810)

JNK1: a protein kinase of the MAPK family that is potently activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, pro-inflammatory cytokines and, in some instances, by growth factors and GPCR agonists. Substrates include a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2, thus regulating AP-1 transcriptional activity. In T- cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. Binds to at least four scaffolding proteins, JIP-1, -2, -3 and -4. Activity increased in obesity. Inhibition or mouse knockout increases insulin sensitivity. Part of NFB pathway involved in inflammation and cancer, and signals downstream of Ras, though possibly as an apoptotic negative regulator of growth. Four alternatively-spliced isoforms have been described. Protein type: Kinase, protein; EC 2.7.11.24; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; CMGC group; MAPK family; JNK subfamily; MAPK/JNK subfamily. Cellular Component: dendrite cytoplasm; mitochondrion; axon; cytoplasm; perikaryon; nucleus; cytosol; vesicle. Molecular Function: MAP kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; histone deacetylase regulator activity; enzyme binding; histone deacetylase binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; JUN kinase activity; ATP binding; protein kinase activity. Biological Process: positive regulation of microtubule polymerization; rhythmic process; signal transduction; protein amino acid phosphorylation; regulation of protein localization; regulation of transcription, DNA-dependent; JNK cascade; inflammatory response; negative regulation of protein binding; neurite development; response to UV; regulation of histone deacetylation; ossification; peptidyl-threonine phosphorylation; determination of dorsal identity; response to osmotic stress; regulation of circadian rhythm; JUN phosphorylation; peptidyl-serine phosphorylation; response to cadmium ion; response to hydrogen peroxide; response to heat; regulation of gene expression; phosphorylation; positive regulation of DNA replication; negative regulation of apoptosis; positive regulation of cell migration

48-Strip-Wells

290 USD

96-Strip-Wells

450