catalog number :
MBS2602635
products type :
ELISA Kit
products full name :
Human Glutamate dehydrogenase 2, mitochondrial, GLUD2 ELISA Kit
products short name :
Glutamate dehydrogenase 2, mitochondrial, GLUD2
other names :
glutamate dehydrogenase 2, mitochondrial; Glutamate dehydrogenase 2, mitochondrial; glutamate dehydrogenase 2, mitochondrial; GDH 2; glutamate dehydrogenase pseudogene 1; glutamate dehydrogenase 2
products gene name :
GLUD2
other gene names :
GLUD2; GLUD2; GDH2; GLUDP1; GLUDP1; GDH 2
uniprot entry name :
DHE4_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant GLUD2 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human GLUD2 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_036216.2
ncbi gb acc num :
NM_012084.3
ncbi mol weight :
61,434 Da
ncbi pathways :
Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); D-Glutamine And D-glutamate Metabolism Pathway (82971); D-Glutamine And D-glutamate Metabolism Pathway (340); GABA Shunt Pathway (139145); Metabolic Pathways (132956); Nitrogen Metabolism Pathway (83025); Nitrogen Metabolism Pathway (416)
ncbi summary :
The protein encoded by this gene is localized to the mitochondrion and acts as a homohexamer to recycle glutamate during neurotransmission. The encoded enzyme catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate. This gene is intronless.[provided by RefSeq, Jan 2010]
uniprot summary :
GLUD2: Important for recycling the chief excitatory neurotransmitter, glutamate, during neurotransmission. Homohexamer. Expressed in retina, testis and, at a lower level, brain. Belongs to the Glu/Leu/Phe/Val dehydrogenases family. Protein type: Mitochondrial; Oxidoreductase; Energy Metabolism - nitrogen; Amino Acid Metabolism - alanine, aspartate and glutamate; Other Amino Acids Metabolism - D-Glutamine and D-glutamate; Amino Acid Metabolism - arginine and proline; EC 1.4.1.3. Chromosomal Location of Human Ortholog: Xq24-q25. Cellular Component: mitochondrion; cytoplasm. Molecular Function: glutamate dehydrogenase activity; glutamate dehydrogenase [NAD(P)+] activity; GTP binding; ADP binding. Biological Process: glutamate catabolic process; glutamate metabolic process; glutamate biosynthetic process