catalog number :
MBS2602629
products type :
ELISA Kit
products full name :
Human Sphingosine, SPH ELISA Kit
products short name :
Sphingosine, SPH
other names :
sphingomyelinase C; Sphingomyelinase C; sphingomyelinase C; Sphingomyelinase C
other gene names :
spH; spH; BCE_0745
uniprot entry name :
Q73DG7_BACC1
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant SPH concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human SPH monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_977070.1
ncbi gb acc num :
NC_003909.8
ncbi mol weight :
42,338 Da
ncbi pathways :
Metabolic Pathways (132135)