catalog number :
MBS2602623
products type :
ELISA Kit
products full name :
Fish Interleukin-2, IL2 ELISA Kit
products short name :
Interleukin-2, IL2
other names :
interleukin-2; Interleukin-2; interleukin-2; TCGF; T-cell growth factor; interleukin-2 precursor protein; T-cell growth factor; TCGF
other gene names :
IL2; IL2; IL-2; IL-2; TCGF
uniprot entry name :
IL2_HORSE
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Fish serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant IL-2 concentration. Assay Type: Sandwich. Detection Range: 1000 pg/ml-15.6 pg/ml. Sensitivity: 5 pg/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Fish IL-2 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_001078902.1
ncbi gb acc num :
NM_001085433.1
ncbi mol weight :
17,086 Da
ncbi pathways :
Allograft Rejection Pathway (90072); Allograft Rejection Pathway (535); Autoimmune Thyroid Disease Pathway (90070); Autoimmune Thyroid Disease Pathway (533); Chagas Disease (American Trypanosomiasis) Pathway (147818); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine-cytokine Receptor Interaction Pathway (90004); Cytokine-cytokine Receptor Interaction Pathway (460); Graft-versus-host Disease Pathway (90073); Graft-versus-host Disease Pathway (536)
uniprot summary :
Function: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.