catalog number :
MBS2602618
products type :
ELISA Kit
products full name :
Pig dermatan sulfate, DS ELISA Kit
products short name :
dermatan sulfate, DS
other names :
dermatan-sulfate epimerase; Dermatan-sulfate epimerase; dermatan-sulfate epimerase; DS epimerase; chondroitin-glucuronate 5-epimerase; squamous cell carcinoma antigen recognized by T-cells 2; dermatan sulfate epimerase; Chondroitin-glucuronate 5-epimerase; Squamous cell carcinoma antigen recognized by T-cells 2; SART-2
other gene names :
DSE; DSE; DSEP; DSEPI; SART2; EDSMC2; SART-2; SART2; DS epimerase; SART-2
uniprot entry name :
DSE_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Porcine serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant DS concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine DS monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_001074445.1
ncbi gb acc num :
NM_001080976.1
ncbi mol weight :
109,773 Da
ncbi pathways :
Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (645308); Dermatan Sulfate Biosynthesis Pathway (645310); Disease Pathway (530764); Glycogen Storage Diseases Pathway (980468); Glycosaminoglycan Biosynthesis - Chondroitin Sulfate / Dermatan Sulfate Pathway (82982); Glycosaminoglycan Biosynthesis - Chondroitin Sulfate / Dermatan Sulfate Pathway (356); Glycosaminoglycan Metabolism Pathway (645297); MPS I - Hurler Syndrome Pathway (685537); MPS II - Hunter Syndrome Pathway (685538); MPS IIIA - Sanfilippo Syndrome A Pathway (685539)
ncbi summary :
The protein encoded by this gene is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is localized to the endoplasmic reticulum. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
uniprot summary :
SART2: Converts D-glucuronic acid to L-iduronic acid (IdoUA) residues. Belongs to the dermatan-sulfate isomerase family. Protein type: Endoplasmic reticulum; Glycan Metabolism - chondroitin sulfate biosynthesis; Isomerase; Membrane protein, integral; Membrane protein, multi-pass; EC 5.1.3.19. Chromosomal Location of Human Ortholog: 6q22. Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum; integral to membrane. Molecular Function: chondroitin-glucuronate 5-epimerase activity. Biological Process: chondroitin sulfate metabolic process; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; heparan sulfate proteoglycan biosynthetic process; carbohydrate metabolic process; pathogenesis; dermatan sulfate biosynthetic process. Disease: Ehlers-danlos Syndrome, Musculocontractural Type 2