catalog number :
MBS260226
products type :
ELISA Kit
products full name :
Rat Interleukin 20 (IL-20) ELISA Kit
products short name :
Interleukin 20 (IL-20)
other names :
Interleukin 20; Interleukin-20; interleukin-20; cytokine Zcyto10; four alpha helix cytokine; interleukin 20; Cytokine Zcyto10
products gene name :
IL-20
other gene names :
IL20; IL20; IL-20; IL10D; ZCYTO10; ZCYTO10; UNQ852/PRO1801; IL-20
uniprot entry name :
IL20_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant IL-20 concentration. Assay Type: Sandwich. Detection Range: 1000 pg/ml-15.6 pg/ml. Sensitivity: 5 pg/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat IL-20 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
AAI17391.1
ncbi mol weight :
17,144 Da
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488)
ncbi summary :
The protein encoded by this gene is a cytokine structurally related to interleukin 10 (IL10). This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis. [provided by RefSeq, Jul 2008]
uniprot summary :
IL20: Cytokine that may be involved in epidermal function and psoriasis. Acts through STAT3. Belongs to the IL-10 family. Protein type: Cytokine; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q32. Cellular Component: extracellular space; extracellular region. Molecular Function: interleukin-20 receptor binding; cytokine activity; interleukin-22 receptor binding. Biological Process: positive regulation of osteoclast differentiation; positive regulation of epidermal cell differentiation; regulation of inflammatory response; positive regulation of JAK-STAT cascade; immune response; inflammatory response; positive regulation of keratinocyte differentiation; positive regulation of tyrosine phosphorylation of Stat3 protein