ELISA/assay

Human Nerve Growth Factor Receptor (NGFR) ELISA kit

MBS260206

48-Strip-Wells

275 USD

ELISA Kit

Human Nerve Growth Factor Receptor (NGFR) ELISA kit

Nerve Growth Factor Receptor (NGFR)

nerve growth factor receptor; Tumor necrosis factor receptor superfamily member 16; tumor necrosis factor receptor superfamily member 16; p75 ICD; NGF receptor; TNFR superfamily, member 16; low affinity nerve growth factor receptor; low affinity neurotrophin receptor p75NTR; low-affinity nerve growth factor receptor; nerve growth factor receptor; Gp80-LNGFR; Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor; NGF receptor; p75 ICD; CD_antigen: CD271

NGFR

NGFR; NGFR; CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR; TNFRSF16; NGF receptor

Human

427

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant NGFR concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human NGFR monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

189205

AAB59544.1

Axonal Growth Inhibition (RHOA Activation) Pathway (106454); Axonal Growth Stimulation Pathway (106453); BDNF Signaling Pathway (712093); Cell Death Signalling Via NRAGE, NRIF And NADE Pathway (106443); Ceramide Signalling Pathway (106451); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); NADE Modulates Death Signalling Pathway (106446); NF-kB Is Activated And Signals Survival Pathway (106450); NFG And ProNGF Binds To P75NTR Pathway (106442)

Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. [provided by RefSeq, Jul 2008]

NGFR: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Homodimer; disulfide-linked. Interacts with p75NTR- associated cell death executor. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1 and NGFRAP1/BEX3. Interacts with KIDINS220 and NTRK1. Can form a ternary complex with NTRK1 and KIDINS220 and this complex is affected by the expression levels of KIDINS220. An increase in KIDINS220 expression leads to a decreased association of NGFR and NTRK1. Interacts with NTRK2; may regulate the ligand specificity of the NTRK2 receptor. Interacts with LINGO1. Protein type: Receptor, misc.; Membrane protein, integral. Chromosomal Location of Human Ortholog: 17q21-q22. Cellular Component: nucleoplasm; cell surface; integral to plasma membrane; extracellular region; plasma membrane; cytosol; endosome. Molecular Function: protein binding; signal transducer activity; transmembrane receptor activity; ubiquitin protein ligase binding; death receptor activity; nerve growth factor binding; receptor activity; Rab GTPase binding. Biological Process: axon guidance; detection of temperature stimulus; central nervous system development; membrane protein intracellular domain proteolysis; hair follicle morphogenesis; nerve growth factor receptor signaling pathway; negative regulation of fibroblast growth factor receptor signaling pathway; positive regulation of apoptosis; positive regulation of odontogenesis of dentine-containing teeth; regulation of axonogenesis; regulation of caspase activity; glucose homeostasis; negative regulation of hair follicle development; negative regulation of cell cycle; intracellular protein transport; positive regulation of fibroblast proliferation; positive regulation of axonogenesis; negative regulation of axonogenesis; nerve development; circadian regulation of gene expression; negative regulation of apoptosis

48-Strip-Wells

275 USD

96-Strip-Wells

420