ELISA/assay

Rat Thymic Stromal Lymphopoietin (TSLP) ELISA Kit

MBS260192

48-Strip-Wells

290 USD

ELISA Kit

Rat Thymic Stromal Lymphopoietin (TSLP) ELISA Kit

Thymic Stromal Lymphopoietin (TSLP)

thymic stromal lymphopoietin isoform 1; Thymic stromal lymphopoietin; thymic stromal lymphopoietin; thymic stromal lymphopoietin

TSLP

TSLP; TSLP

TSLP_HUMAN

Rat

159

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Rat serum, plasma or cell culture supernatant and organizations in the natural and recombinant TSLP concentration. Assay Type: Sandwich. Detection Range: 1000 pg/ml-15.6 pg/ml. Sensitivity: 5 pg/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat TSLP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

14719428

NP_149024.1

NM_033035.4

18,141 Da

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); TSLP Signaling Pathway (672451)

This gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]

TSLP: Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 5q22.1. Cellular Component: extracellular space. Molecular Function: cytokine activity

48-Strip-Wells

290 USD

96-Strip-Wells

450