ELISA/assay

Human Interferon-induced GTP-binding protein Mx1 (MX1) ELISA Kit

MBS2601914

48-Strip-Wells

275 USD

ELISA Kit

Human Interferon-induced GTP-binding protein Mx1 (MX1) ELISA Kit

Interferon-induced GTP-binding protein Mx1 (MX1)

interferon-induced GTP-binding protein Mx1 isoform b; Interferon-induced GTP-binding protein Mx1; interferon-induced GTP-binding protein Mx1; myxoma resistance protein 1; interferon-induced protein p78; interferon-inducible protein p78; interferon-regulated resistance GTP-binding protein MxA; myxovirus (influenza virus) resistance 1, interferon-inducible protein p78; MX dynamin-like GTPase 1; Interferon-induced protein p78; IFI-78K; Interferon-regulated resistance GTP-binding protein MxA; Myxoma resistance protein 1; Myxovirus resistance protein 1Cleaved into the following chain:Interferon-induced GTP-binding protein Mx1, N-terminally processed

MX1

MX1; MX1; MX; MxA; IFI78; IFI-78K; IFI-78K

MX1_HUMAN

Human

508

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant MX1 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human MX1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

544711185

NP_001269849.1

NM_001282920.1

55,661 Da

Antiviral Mechanism By IFN-stimulated Genes Pathway (530760); Cytokine Signaling In Immune System Pathway (366171); ISG15 Antiviral Mechanism Pathway (530761); Immune System Pathway (106386); Influenza A Pathway (217173); Influenza A Pathway (217150); Interferon Signaling Pathway (187103); Interferon Alpha/beta Signaling Pathway (187104); Measles Pathway (213306); Measles Pathway (213277)

This gene encodes a guanosine triphosphate (GTP)-metabolizing protein that participates in the cellular antiviral response. The encoded protein is induced by type I and type II interferons and antagonizes the replication process of several different RNA and DNA viruses. There is a related gene located adjacent to this gene on chromosome 21, and there are multiple pseudogenes located in a cluster on chromosome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

MX1: Dynamin-like GTPase acting as a cell-autonomous host restriction factor against many viral pathogens. Shows activity against influenza viruses, rhabdovirus VSV, bunyavirus LACV, Thogoto virus, measles virus, Hantaan virus, Coxsackie virus CVB, Rift Valley fever virus RVFV, hepatitis B virus HBV, and Crimean- Congo hemorrhagic fever virus CCHFV. Belongs to the dynamin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Hydrolase; Apoptosis. Chromosomal Location of Human Ortholog: 21q22.3. Cellular Component: endoplasmic reticulum membrane; nuclear membrane; perinuclear region of cytoplasm; cytoplasm; cytosol. Molecular Function: GTPase activity; protein binding; GTP binding. Biological Process: negative regulation of viral genome replication; apoptosis; metabolic process; response to virus; cytokine and chemokine mediated signaling pathway; innate immune response; defense response; signal transduction; defense response to virus

48-Strip-Wells

275 USD

96-Strip-Wells

420