ELISA/assay

Rabbit Macrophage Inflammatory Protein 1 Alpha (MIP-1alpha) ELISA kit

MBS2601749

48-Strip-Wells

275 USD

ELISA Kit

Rabbit Macrophage Inflammatory Protein 1 Alpha (MIP-1alpha) ELISA kit

[Macrophage Inflammatory Protein 1 Alpha (MIP-1alpha)]

[Rabbit Macrophage Inflammatory Protein 1 Alpha (MIP-1a)  ELISA kit]

[macrophage inflammatory protein 1 alpha, partial; C-C motif chemokine 3; C-C motif chemokine 3; SIS-beta; PAT 464.1; G0/G1 switch regulatory protein 19-1; macrophage inflammatory protein 1-alpha; tonsillar lymphocyte LD78 alpha protein; small inducible cytokine A3 (homologous to mouse Mip-1a); chemokine (C-C motif) ligand 3; G0/G1 switch regulatory protein 19-1; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; PAT 464.1; SIS-beta; Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha proteinCleaved into the following chain:MIP-1-alpha(4-69)Alternative name(s):LD78-alpha(4-69)]

[MIP-1alpha]

[CCL3; CCL3; MIP1A; SCYA3; G0S19-1; LD78ALPHA; MIP-1-alpha; G0S19-1; MIP1A; SCYA3; MIP-1-alpha]

CCL3_HUMAN

Rabbit

70

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Rabbit Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Mc Concentration. Assay Type: Sandwich. Detection Range: 100 ng/ml-1.56 ng/ml. Sensitivity: 0.5 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rabbit MC monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

2905628

AAC03539.1

10,085 Da

Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); GPCR Ligand Binding Pathway (161020); IL12-mediated Signaling Events Pathway (137922)

This locus represents a small inducible cytokine. The encoded protein, also known as macrophage inflammatory protein 1 alpha, plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. Polymorphisms at this locus may be associated with both resistance and susceptibility to infection by human immunodeficiency virus type 1.[provided by RefSeq, Sep 2010]

CCL3: Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Belongs to the intercrine beta (chemokine CC) family. Protein type: Motility/polarity/chemotaxis; Secreted; Chemokine; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 17q12. Cellular Component: extracellular space; cytoplasm; extracellular region; intracellular; cytosol. Molecular Function: identical protein binding; CCR1 chemokine receptor binding; protein binding; chemokine activity; calcium-dependent protein kinase C activity; kinase activity; phospholipase activator activity; CCR5 chemokine receptor binding; protein kinase activity; chemoattractant activity. Biological Process: positive regulation of catalytic activity; negative regulation of osteoclast differentiation; exocytosis; behavior; response to toxin; positive regulation of calcium-mediated signaling; positive regulation of interleukin-1 beta secretion; chemotaxis; protein amino acid phosphorylation; negative regulation of bone mineralization; regulation of cell shape; monocyte chemotaxis; cell-cell signaling; positive chemotaxis; positive regulation of neuron apoptosis; calcium ion transport; lipopolysaccharide-mediated signaling pathway; protein kinase B signaling cascade; inflammatory response; lymphocyte chemotaxis; neutrophil chemotaxis; calcium-mediated signaling; MAPKKK cascade; cytoskeleton organization and biogenesis; positive regulation of tumor necrosis factor production; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; macrophage chemotaxis; osteoblast differentiation; cellular calcium ion homeostasis; positive regulation of protein kinase B signaling cascade; cell activation; eosinophil chemotaxis; eosinophil degranulation; regulation of sensory perception of pain; positive regulation of calcium ion transport; astrocyte cell migration; positive regulation of cell migration; positive regulation of inflammatory response. Disease: Human Immunodeficiency Virus Type 1, Susceptibility To

48-Strip-Wells

275 USD

96-Strip-Wells

435

5x96-Strip-Wells

1755

10x96-Strip-Wells

3150