catalog number :
MBS2601406
products type :
ELISA Kit
products full name :
Rat Hypoxia Inducible Factor 2 Alpha (HIF-2alpha) ELISA Kit
products short name :
[Hypoxia Inducible Factor 2 Alpha (HIF-2alpha)]
products name syn :
[Rat Hypoxia Inducible Factor 2 Alpha (HIF-2a) ELISA Kit]
other names :
[hypoxia inducible factor 2 alpha; Endothelial PAS domain-containing protein 1; endothelial PAS domain-containing protein 1; EPAS-1; HIF2 alpha; HIF2-alpha; HIF-2 alpha; HIF-2-alpha; hypoxia inducible factor 2a; hypoxia-inducible factor 2 alpha; hypoxia-inducible factor 2-alpha; hypoxia inducible factor 2, alpha subunit; endothelial PAS domain protein 1; Hypoxia-inducible factor 2-alpha; HIF-2-alpha; HIF2-alpha]
products gene name :
[HIF-2alpha]
other gene names :
[Epas1; Epas1; HLF; Hif2a; Hif2a; EPAS-1; HIF-2-alpha; HIF2-alpha]
uniprot entry name :
EPAS1_RAT
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Rat Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Hgf Concentration. Assay Type: Sandwich. Detection Range: 1000 pg/ml-15.6 pg/ml. Sensitivity: 5 pg/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat HGF monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
CAB96612.1
ncbi mol weight :
96,718 Da
ncbi pathways :
Adipogenesis Pathway (198479); Pathways In Cancer (83493); Renal Cell Carcinoma Pathway (83495); Renal Cell Carcinoma Pathway (519)
ncbi summary :
activates luciferase activity after transfection [RGD, Feb 2006]
uniprot summary :
Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD ().
size4 :
10x96-Strip-Wells