ELISA/assay

Goat 1,25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit

MBS2600890

96 Strip Wells

490 USD

ELISA Kit

Goat 1,25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit

1,25-Dihydroxyvitamin D3 (DHVD3)

1,25-dihydroxyvitamin D3 24-hydroxylase; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 24-OHase; cytochrome P450 24A1; cytochrome P450-CC24; vitamin D 24-hydroxylase; exo-mitochondrial protein; vitamin D(3) 24-hydroxylase; 1,25-@dihydroxyvitamin D3 24-hydroxylase; cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase); cytochrome P450, family 24, subfamily A, polypeptide 1; Cytochrome P450 24A1; Cytochrome P450-CC24

DHVD3

CYP24A1; CYP24A1; CP24; HCAI; CYP24; P450-CC24; CYP24; 24-OHase; Vitamin D(3) 24-hydroxylase

CP24A_HUMAN

Goat

513

Specificity No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: This product is suitable for in vitro quantitative detection of Goat serum, plasma or cell culture supernatant and organizations in the natural and recombinant DHVD3 concentration. Detection Range: 10 ng/ml-0.156 ng/ml

Sensitivity: the minimum detectable Goat DHVD3 up to 0.05 ng/ml. Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Goat DHVD3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

306704

AAA62379.1

43,051 Da

Biological Oxidations Pathway 105698!!Cytochrome P450 - Arranged By Substrate Type Pathway 105700!!Metabolic Pathways 132956!!Metabolism Pathway 477135!!Metabolism Of Lipids And Lipoproteins Pathway 160976!!Metabolism Of Steroid Hormones And Vitamin D Pathway 106150!!MicroRNAs In Cancer Pathway 852705!!MicroRNAs In Cancer Pathway 852928!!Nuclear Receptors In Lipid Metabolism And Toxicity Pathway 198887!!Phase 1 - Functionalization Of Compounds Pathway 105699

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

96 Strip Wells

490 USD