ELISA/assay

Human Aldose Reductase (AR) ELISA Kit

MBS2600759

48-Strip-Wells

275 USD

ELISA Kit

Human Aldose Reductase (AR) ELISA Kit

Aldose Reductase (AR)

aldose reductase; Aldose reductase; aldose reductase; aldehyde reductase 1; low Km aldose reductase; Lii5-2 CTCL tumor antigen; aldo-keto reductase family 1 member B1; aldo-keto reductase family 1, member B1 (aldose reductase); Aldehyde reductase; Aldo-keto reductase family 1 member B1

AR

AKR1B1; AKR1B1; AR; ADR; ALR2; ALDR1; ALDR1; AR

ALDR_HUMAN

Human

316

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant AR concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human AR monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

4502049

NP_001619.1

NM_001628.2

35,853 Da

Fructose And Mannose Metabolism Pathway (82930); Fructose And Mannose Metabolism Pathway (291); Galactose Metabolism Pathway (82931); Galactose Metabolism Pathway (292); Glycerolipid Metabolism Pathway (82986); Glycerolipid Metabolism Pathway (361); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Metabolism Of Steroid Hormones And Vitamin D Pathway (106150)

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq, Feb 2009]

AKR1B1: Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. Belongs to the aldo/keto reductase family. Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - pentose and glucuronate interconversions; Carbohydrate Metabolism - pyruvate; Oxidoreductase; EC 1.1.1.21; Carbohydrate Metabolism - galactose; Lipid Metabolism - glycerolipid. Chromosomal Location of Human Ortholog: 7q35. Cellular Component: nucleoplasm; extracellular space; cytoplasm; cytosol. Molecular Function: glyceraldehyde oxidoreductase activity; electron carrier activity; aldo-keto reductase activity; aldehyde reductase activity. Biological Process: steroid metabolic process; carbohydrate metabolic process; C21-steroid hormone biosynthetic process; response to stress

48-Strip-Wells

275 USD

96-Strip-Wells

420