Rat Catalase (CAT) ELISA Kit | MyBioSource MBS2600683 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Catalase (CAT) ELISA Kit

catalog :

MBS2600683

quantity :

48-Strip-Wells

price :

290 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2600683

products type :

ELISA Kit

products full name :

Rat Catalase (CAT) ELISA Kit

products short name :

Catalase (CAT)

other names :

catalase; Catalase; catalase; catalase

products gene name :

CAT

other gene names :

CAT; CAT

uniprot entry name :

CATA_HUMAN

reactivity :

Rat

sequence length :

527

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant CAT concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat CAT monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

4557014

ncbi acc num :

NP_001743.1

ncbi gb acc num :

NM_001752.3

ncbi mol weight :

59,756 Da

ncbi pathways :

Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Cellular Responses To Stress Pathway (645258); DNA Damage Response (only ATM Dependent) Pathway (198827); Detoxification Of Reactive Oxygen Species Pathway (941400); Folate Metabolism Pathway (198833); FoxO Family Signaling Pathway (138036); FoxO Signaling Pathway (921162); Glyoxylate And Dicarboxylate Metabolism Pathway (83002)

ncbi summary :

This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq, Oct 2009]

uniprot summary :

Catalase: Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. Homotetramer. Belongs to the catalase family. Protein type: Apoptosis; Mitochondrial; EC 1.11.1.6; Amino Acid Metabolism - tryptophan; Energy Metabolism - methane; Oxidoreductase; Endoplasmic reticulum; Hydrolase. Chromosomal Location of Human Ortholog: 11p13. Cellular Component: Golgi apparatus; peroxisomal membrane; peroxisomal matrix; focal adhesion; intracellular membrane-bound organelle; membrane; lysosome; endoplasmic reticulum; plasma membrane; mitochondrial intermembrane space; peroxisome; cytosol. Molecular Function: antioxidant activity; oxidoreductase activity, acting on peroxide as acceptor; enzyme binding; protein homodimerization activity; catalase activity; metal ion binding; heme binding; NADP binding; receptor binding; aminoacylase activity. Biological Process: cholesterol metabolic process; hemoglobin metabolic process; nucleobase, nucleoside and nucleotide metabolic process; purine base metabolic process; protein homotetramerization; activation of NF-kappaB transcription factor; positive regulation of phosphoinositide 3-kinase cascade; osteoblast differentiation; response to vitamin E; response to reactive oxygen species; response to hyperoxia; triacylglycerol metabolic process; UV protection; ureteric bud development; inhibition of NF-kappaB transcription factor; hydrogen peroxide catabolic process; positive regulation of cell division; aerobic respiration; response to hypoxia; purine nucleotide catabolic process; protein tetramerization; negative regulation of apoptosis; aging. Disease: Acatalasemia

size1 :

48-Strip-Wells

price1 :

290 USD

size2 :

96-Strip-Wells

price2 :

450

more info or order :

MyBioSource product webpage