ELISA/assay

Mouse Bisphenol A (BPA) ELISA Kit

MBS2600653

48-Strip-Wells

290 USD

ELISA Kit

Mouse Bisphenol A (BPA) ELISA Kit

Bisphenol A (BPA)

bisphenol A receptor ERRgamma2; Estrogen-related receptor gamma; estrogen-related receptor gamma; ERR gamma-2; estrogen receptor-related protein 3; nuclear receptor subfamily 3 group B member 3; estrogen-related receptor gamma; ERR gamma-2; Estrogen receptor-related protein 3; Nuclear receptor subfamily 3 group B member 3

BPA

ESRRG; ESRRG; ERR3; NR3B3; ERRgamma; ERR3; ERRG2; KIAA0832; NR3B3

ERR3_HUMAN

Mouse

435

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant BPA concentration. Assay Type: Sandwich. Detection Range: 200 ng/ml-3.12 ng/ml. Sensitivity: 0.6 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse BPA monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

226000901

BAH36764.1

52,462 Da

Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Nuclear Receptor Transcription Pathway (105979)

This gene encodes a member of the estrogen receptor-related receptor (ESRR) family, which belongs to the nuclear hormone receptor superfamily. All members of the ESRR family share an almost identical DNA binding domain, which is composed of two C4-type zinc finger motifs. The ESRR members are orphan nuclear receptors; they bind to the estrogen response element and steroidogenic factor 1 response element, and activate genes controlled by both response elements in the absence of any ligands. The ESRR family is closely related to the estrogen receptor (ER) family. They share target genes, co-regulators and promoters, and by targeting the same set of genes, the ESRRs seem to interfere with the ER-mediated estrogen response in various ways. It has been reported that the family member encoded by this gene functions as a transcriptional activator of DNA cytosine-5-methyltransferases 1 (Dnmt1) expression by direct binding to its response elements in the DNMT1 promoters, modulates cell proliferation and estrogen signaling in breast cancer, and negatively regulates bone morphogenetic protein 2-induced osteoblast differentiation and bone formation. Multiple alternatively spliced transcript variants have been identified, which mainly differ at the 5' end and some of which encode protein isoforms differing in the N-terminal region. [provided by RefSeq, Aug 2011]

ERR3: Orphan receptor that acts as transcription activator in the absence of bound ligand. Binds specifically to an estrogen response element and activates reporter genes controlled by estrogen response elements. Belongs to the nuclear hormone receptor family. NR3 subfamily. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Transcription factor; Nuclear receptor; DNA-binding. Chromosomal Location of Human Ortholog: 1q41. Cellular Component: nucleoplasm. Molecular Function: protein binding; zinc ion binding; AF-2 domain binding; steroid hormone receptor activity; retinoic acid receptor activity; steroid binding. Biological Process: retinoic acid receptor signaling pathway; transcription initiation from RNA polymerase II promoter; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; gene expression; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter

48-Strip-Wells

290 USD

96-Strip-Wells

450