ELISA/assay

Human Estrogen Receptor Alpha (ERalpha) ELISA Kit

MBS2600479

48-Strip-Wells

275 USD

ELISA Kit

Human Estrogen Receptor Alpha (ERalpha) ELISA Kit

Estrogen Receptor Alpha (ERalpha)

Human Estrogen Receptor Alpha (ERa) ELISA Kit

estrogen receptor alpha, partial; Estrogen receptor; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1

ERalpha

ESR1; ESR; NR3A1; ER

ESR1_HUMAN

Human

444

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant ERalpha concentration. Assay Type: Sandwich. Detection Range: 40 ng/ml-0.625 ng/ml. Sensitivity: 0.1ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human ERalpha monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

5821726

AAD52984.1

35,547 Da

ER-alpha: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described. Protein type: DNA-binding; Nuclear receptor. Chromosomal Location of Human Ortholog: 6q25.1. Cellular Component: nucleoplasm; Golgi apparatus; membrane; cytoplasm; plasma membrane; integral to membrane; nucleus. Molecular Function: identical protein binding; estrogen receptor activity; zinc ion binding; nitric-oxide synthase regulator activity; beta-catenin binding; estrogen response element binding; transcription factor binding; steroid binding; protein binding; enzyme binding; steroid hormone receptor activity; chromatin binding; transcription factor activity. Biological Process: positive regulation of nitric oxide biosynthetic process; estrogen receptor signaling pathway; signal transduction; uterus development; response to estradiol stimulus; regulation of apoptosis; positive regulation of fibroblast proliferation; elevation of cytosolic calcium ion concentration; regulation of transcription, DNA-dependent; epithelial cell development; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; antral ovarian follicle growth; negative regulation of transcription factor activity; positive regulation of nitric-oxide synthase activity; androgen metabolic process; male gonad development; vagina development; positive regulation of retinoic acid receptor signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; chromatin remodeling; response to estrogen stimulus; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); gene expression; positive regulation of transcription factor activity. Disease: Migraine With Or Without Aura, Susceptibility To, 1; Breast Cancer; Myocardial Infarction, Susceptibility To; Estrogen Resistance

48-Strip-Wells

275 USD

96-Strip-Wells

420