catalog number :
MBS2600382
products type :
ELISA Kit
products full name :
Mouse Beta-Defensins 3 (DEFbeta3) ELISA Kit
products short name :
[Beta-Defensins 3 (DEFbeta3)]
products name syn :
[Mouse Beta-Defensins 3 (DEFb3) ELISA Kit]
other names :
[beta-defensin 103; Beta-defensin 103; beta-defensin 103; beta-defensin 3; defensin, beta 103; defensin-like protein; defensin, beta 103B; Beta-defensin 3; BD-3; DEFB-3; HBD3; hBD-3; Defensin, beta 103; Defensin-like protein]
products gene name :
[DEFbeta3]
other gene names :
[DEFB103B; DEFB103A; BD-3; HBD3; HBP3; DEFB3; HBD-3; HBP-3; DEFB-3; DEFB103; BD3; DEFB103; DEFB3 DEFB103B ; BD-3; DEFB-3; HBD3; hBD-3]
uniprot entry name :
D103A_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Mouse serum, plasma or cell culture supernatant and organizations in the natural and recombinant DEFbeta3 concentration. Detection Range: 2000 pg/ml-31.2 pg/ml. Sensitivity: Up to 12 pg/ml. Assay Type: Sandwich
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse DEFbeta3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_061131.1
ncbi gb acc num :
NM_018661.4
ncbi mol weight :
7,697 Da
ncbi pathways :
Beta Defensins Pathway (530759); Defensins Pathway (530757); Immune System Pathway (106386); Innate Immune System Pathway (106387)
ncbi summary :
Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 103B, which has broad spectrum antimicrobial activity and may play an important role in innate epithelial defense. [provided by RefSeq, Jul 2008]
uniprot summary :
defensin, beta 3: Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistant E.faecium. No significant hemolytic activity was observed. Belongs to the beta-defensin family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 8p23.1. Cellular Component: Golgi lumen; extracellular region. Biological Process: defense response to bacterium; innate immune response; positive regulation of biosynthetic process of antibacterial peptides active against Gram-positive bacteria
size4 :
10x96-Strip-Wells