ELISA/assay

Human Transferrin (TRF) ELISA Kit

MBS2600168

48-Strip-Wells

250 USD

ELISA Kit

Human Transferrin (TRF) ELISA Kit

[Transferrin (TRF)]

[transferrin; Serotransferrin; serotransferrin; siderophilin; beta-1 metal-binding globulin; transferrin; Beta-1 metal-binding globulin; Siderophilin]

[TRF]

[TF; TF; TFQTL1; PRO1557; PRO2086; PRO1400; Transferrin]

TRFE_HUMAN

Human

698

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma or cell culture supernatant and organizations in the natural and recombinant TRF concentration. Assay Type: Sandwich. Detection Range: 100 ng/ml-1.56 ng/ml. Sensitivity: 0.5 ng/ml.

Intra-assay Precision: ? 8%. Inter-assay Precision: ? 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human TRF monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

339453

AAA61140.1

77,064 Da

EPHB Forward Signaling Pathway (138047); HIF-1 Signaling Pathway (695200); HIF-1-alpha Transcription Factor Network Pathway (138045); Hemostasis Pathway (106028); Iron Metabolism In Placenta Pathway (672461); Iron Uptake And Transport Pathway (187191); Mineral Absorption Pathway (212237); Mineral Absorption Pathway (212220); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050)

This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq, Sep 2009]

TF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. Defects in TF are the cause of atransferrinemia (ATRAF). Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. Belongs to the transferrin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 3q22.1. Cellular Component: extracellular space; recycling endosome; cell surface; endocytic vesicle; perinuclear region of cytoplasm; early endosome; late endosome; apical plasma membrane; cytoplasmic membrane-bound vesicle; basal plasma membrane; extracellular region; coated pit; endosome membrane; vesicle. Molecular Function: protein binding; ferric iron binding; ubiquitin protein ligase binding. Biological Process: platelet activation; retinal homeostasis; platelet degranulation; cellular iron ion homeostasis; transferrin transport; blood coagulation; transmembrane transport. Disease: Atransferrinemia

48-Strip-Wells

250 USD

96-Strip-Wells

365

5x96-Strip-Wells

1480

10x96-Strip-Wells

2620