ELISA/assay

Human Carbohydrate Sulfotransferase 15 (CHST15) ELISA Kit

MBS2600006

48-Strip-Wells

250 USD

ELISA Kit

Human Carbohydrate Sulfotransferase 15 (CHST15) ELISA Kit

[Carbohydrate Sulfotransferase 15 (CHST15)]

[carbohydrate sulfotransferase 15; Carbohydrate sulfotransferase 15; carbohydrate sulfotransferase 15; GalNAc4S-6ST; B-cell RAG-associated gene protein; GalNAc 4-sulfate 6-O-sulfotransferase; N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase; carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15; B-cell RAG-associated gene protein; N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase; GalNAc4S-6ST]

[CHST15]

[Chst15; Chst15; BRAG; MAd5; mKIAA0598; GalNAcS-6ST; 4631426J05Rik; Brag; Galnac4s6st; Kiaa0598; GalNAc4S-6ST]

CHSTF_MOUSE

Human

561

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Human Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Chem Concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human CHEM monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

31560040

NP_084211.2

NM_029935.5

64,986 Da

B Cell Receptor Signaling Pathway (198285); Chondroitin Sulfate Biosynthesis Pathway (1000658); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (1000657); Disease Pathway (970606); Glycogen Storage Diseases Pathway (970607); Glycosaminoglycan Biosynthesis - Chondroitin Sulfate / Dermatan Sulfate Pathway (83181); Glycosaminoglycan Biosynthesis - Chondroitin Sulfate / Dermatan Sulfate Pathway (356); Glycosaminoglycan Metabolism Pathway (1000645); MPS I - Hurler Syndrome Pathway (1000644); MPS II - Hunter Syndrome Pathway (1000661)

CHST15: Sulfotransferase that transfers sulfate from 3 - phosphoadenosine 5 -phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non- reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B- cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo. Belongs to the sulfotransferase 1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 2.8.2.33; Transferase; Glycan Metabolism - chondroitin sulfate biosynthesis; Membrane protein, integral. Cellular Component: Golgi apparatus; membrane; integral to membrane. Molecular Function: N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase activity; transferase activity; sulfotransferase activity; 3 -phosphoadenosine 5 -phosphosulfate binding. Biological Process: hexose biosynthetic process

48-Strip-Wells

250 USD

96-Strip-Wells

365

5x96-Strip-Wells

1480

10x96-Strip-Wells

2620