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Inflammasome Antibody Sampler Kit

MBS2549977

1 Kit

345 USD

Antibody Kit

Inflammasome Antibody Sampler Kit

[Inflammasome]

Western Blot (WB)

Western Blot (WB)

Western Blot (WB)

Western Blot (WB)

Western Blot (WB)

Western Blot analysis of 293 cells using Caspase-1 Polyclonal Antibody at dilution of 1:500.

Western Blot (WB)

Western Blot analysis of various cells using ASC Polyclonal Antibody at dilution of 1:1000.

Samples: Cell Lysates, Tissue Extracts And Serum. Assay Type: Sandwich. Sensitivity: 3.125ng/mL

Intra-assay Precision: Intra-Assay Precision (Within Run Precision) To determine Intra-Assay Precision, three samples of known concentration were assayed thirty times on one plate. The Intra-Assay Coefficient of variation of HSP60 (human), EIA has been determined to be <10%. Inter-assay Precision: Inter-Assay Precision (Between Run Precision) To determine Inter-Assay Precision, three samples of known concentration were assayed thirty times in three individual assays. The Inter-Assay Coefficient of variation of the Hsp60 ELISA has been determined to be <10%.

Background/Introduction: The HSP60 (human), EIA kit provides a reliable and sensitive method to detect Hsp60 in cell lysates, tissue extracts or serum. The assay is specific for Hsp60 and does not detect other Hsp60 family members, such as GroEL and Hsp65. HSP60 (human), EIA is a sandwich immunoassay. A mouse monoclonal antibody specific for Hsp60 is pre-coated on the wells of the provided Anti-Hsp60 Immunoassay Plate. Hsp60 is captured by the immobilized antibody and is detected with an Hsp60 specific, goat polyclonal antibody. The goat polyclonal antibody is subsequently bound by a horseradish peroxidase conjugated anti-goat IgG secondary antibody. The assay is developed with tetramethylbenzidine substrate and a blue color develops in proportion to the amount of captured Hsp60. The color development is stopped with acid stop solution which converts the endpoint color to yellow. The intensity of the color is measured in a microplate reader at 450nm. Hsp60 concentrations from the sample are determined by interpolating absorbance readings from a standard curve generated with the calibrated Hsp60 protein standard provided. The traditional method for Hsp60 determination is accomplished in two steps: immunoblotting followed by densitometry scanning. HSP60 (human), EIA provides researchers with a rapid and reliable method to measure the levels of Hsp60 in numerous samples. This kit has the potential of expanding our knowledge of Hsp60's role in critical cellular processes or implicating Hsp60 as a diagnostic tool to evaluate and monitor a variety of diseases.

1 Kit

345 USD