Active Caspase-3 Monoclonal Antibody | MyBioSource MBS2548892 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Active Caspase-3 Monoclonal Antibody

catalog :

MBS2548892

quantity :

0.02 mL

price :

110 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot analysis of 1) Hela, 2) 3T3, 3) Rat brain with Active Caspase-3 Monoclonal Antibody.

image 2 :

Immunohistochemistry of paraffin-embedded Human liver tissue using Active Caspase-3 Monoclonal Antibody at dilution of 1:200.

image 3 :

Immunofluorescence analysis of Rat lung tissue using Active Caspase-3 Monoclonal Antibody at dilution of 1:200.

product information

catalog number :

MBS2548892

products type :

Antibody

products full name :

Active Caspase-3 Monoclonal Antibody

products short name :

[Active Caspase-3]

products name syn :

[CASP3,Caspase 3,apoptosis-related cysteine peptidase,apoptosis-related cysteine protease,. CPP-32,CPP32,CPP32B,Cysteine protease CPP32]

other names :

[caspase-3 isoform a preproprotein; Caspase-3; caspase-3; caspase 3; Apopain; Cysteine protease CPP32; CPP-32]

other gene names :

[CASP3; CASP3; CPP32; SCA-1; CPP32B; CPP32; CASP-3; CPP-32; SCA-1]

clonality :

Monoclonal

isotype :

IgG

host :

Mouse

reactivity :

Human, Mouse, Rat

sequence length :

277

purity :

Protein A Purification

storage stability :

Store at -20°C. Avoid freeze / thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF)

app notes :

WB: 1:500-1:1000. IHC: 1:100-1:200. IF: 1:100-1:200

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Immunogen: Recombinant Protein. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.4. Observed MW: 17kDa

products categories :

Cancer

products description :

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.

ncbi gi num :

14790119

ncbi acc num :

NP_004337.2

ncbi gb acc num :

NM_004346.3

uniprot acc num :

P42574

ncbi pathways :

AGE/RAGE Pathway (698754); Activation Of DNA Fragmentation Factor Pathway (105683); Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway (105672); Alpha6-Beta4 Integrin Signaling Pathway (198807); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099)

ncbi summary :

The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017]

uniprot summary :

CASP3: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family. Protein type: Apoptosis; EC 3.4.22.56; Motility/polarity/chemotaxis; Protease. Chromosomal Location of Human Ortholog: 4q35.1. Cellular Component: cytosol; membrane raft; nucleoplasm; nucleus; plasma membrane. Molecular Function: aspartic-type endopeptidase activity; caspase activator activity; cyclin-dependent protein serine/threonine kinase inhibitor activity; cysteine-type endopeptidase activity; death receptor binding; peptidase activity; phospholipase A2 activator activity; protease binding; protein binding; protein complex binding. Biological Process: activation of cysteine-type endopeptidase activity involved in apoptotic process; activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c; apoptosis; apoptotic DNA fragmentation; B cell homeostasis; cell fate commitment; cellular response to DNA damage stimulus; erythrocyte differentiation; heart development; hippocampus development; intrinsic apoptotic signaling pathway in response to osmotic stress; keratinocyte differentiation; learning and/or memory; negative regulation of activated T cell proliferation; negative regulation of apoptosis; negative regulation of B cell proliferation; negative regulation of cyclin-dependent protein serine/threonine kinase activity; nerve growth factor receptor signaling pathway; neuron apoptosis; neuron differentiation; platelet formation; positive regulation of neuron apoptotic process; protein processing; proteolysis; regulation of macroautophagy; response to amino acid; response to antibiotic; response to cobalt ion; response to drug; response to estradiol; response to glucocorticoid stimulus; response to glucose stimulus; response to hydrogen peroxide; response to hypoxia; response to lipopolysaccharide; response to nicotine; response to tumor necrosis factor; response to UV; response to X-ray; sensory perception of sound; T cell homeostasis; wound healing

size1 :

0.02 mL

price1 :

110 USD

size2 :

0.06 mL

price2 :

150

size3 :

0.12 mL

price3 :

225

size4 :

0.2 mL

price4 :

360

more info or order :

MyBioSource product webpage