catalog number :
MBS2548432
products type :
Assay Kit
products full name :
Total antioxidant capacity Assay Kit (ABTS method)
products short name :
[Total antioxidant capacity]
specificity :
The Lovebird sexing kit is designed for the in vitro detection of the Agapornis Z and W chromosomes; enabling the identification of the bird's sex. The primers and probe sequences in this kit have 100% homology with a broad range of clinically relevant reference sequences based on a comprehensive bioinformatics analysis. They therefore have a very broad detection profile. This kit will detect Agapornis roseicollis and Agapornis personata only. This kit will not detect Psittaculirostris desmarestii and Psephotus haematonotus.
storage stability :
The kit is stable for 6 months at 2-8 degree C before opening.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
products description :
Principle of the Assay: The primers/probes target the spindlin gene that is unique for each of the sex chromosomes and targets the variable region. The kit contains two Agapornis primer and probe sets. The Agapornis_spp-Z primer and probe set are designed to detect both male and female members of the Lovebird species. The Agapornis_spp-W primer and probe set is specific to female lovebirds. Samples that test positive for both the Agapornis_spp-W and Agapornis_spp-Z kits can be confidently predicted to be female. Samples that only produce a strong positive signal for Agapornis_spp-Z are male members of the species. Please refer to page 11 for further information about data interpretation!!Background/Introduction: Lovebirds are small parrots of the genus Agapornis, nine species of lovebird exist with eight being native to Africa and one species native to Madagascar. In the wild these species are separated geographically and possess different unique colourings. Determining the sex of lovebirds is difficult, in certain species colourings are identical between sexes. Behavioural cues can give an idea of sex but are not 100% reliable. Some experts may be able to determine sex by feeling birds beneath the body, however this becomes difficult with extremely young birds. The only reliable option is DNA testing, the rationale of this kit is to test for the Z chromosome and the W chromosome. Males are ZZ whilst females are ZW, this means that by testing for each chromosome individually we can determine the sex of the bird using qPCR.