Recombinant Mouse GFRA1 / GFR alpha-1 Protein (His tag) | MyBioSource MBS2547125 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Mouse GFRA1 / GFR alpha-1 Protein (His tag)

catalog :

MBS2547125

quantity :

0.05 mg

price :

435 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2547125

products type :

Recombinant Protein

products full name :

Recombinant Mouse GFRA1 / GFR alpha-1 Protein (His tag)

products short name :

[GFRA1 / GFR alpha-1]

products name syn :

[AU042498]

other names :

[GDNF receptor beta; GDNF family receptor alpha-1; GDNF family receptor alpha-1; glial cell line derived neurotrophic factor family receptor alpha 1; TGF-beta-related neurotrophic factor receptor 1]

products gene name :

[GFRA1]

other gene names :

[Gfra1; Gfra1; AU042498; Gdnfra; Trnr1; GDNF receptor alpha-1; GDNFR-alpha-1; GFR-alpha-1]

host :

HEK293 Cells

sequence length :

463

sequence :

Met 1-Ser 425

purity :

> 98 % as determined by SDS-PAGE

form :

Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.

storage stability :

Shipping: Blue Ice. Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

app notes :

The secreted recombinant mouse GFRalpha1consists of 412 amino acids and has a calculated molecular mass of 46.3 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 55-60 kDa protein in SDS-PAGE under reducing conditions.

image1 heading :

SDS-PAGE

other info1 :

Species: Mouse. Tag: C-His. Bioactivity: 1. Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 for this effect is typically 0.3-1.5 ug/mL in the presence of 40ng/mL Recombinant Human GDNF. 2. Measured by its ability to bind with human GDNF in a functional ELISA. Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method. Reconstitution: It is recommended that sterile water (330ul) be added to the vial to prepare a stock solution of 0.15mg/mL. Concentration is measured by UV-Vis.

products description :

Glial cell line derived neurotrophic factor (GDNF) Family Receptor Alpha 1 (GFRA1) is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRA1 is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are its binding ligand which are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. The RET is a receptor tyrosine kinase representing the signal-transducing molecule of a multisubunit surface receptor complex for the GDNF, in which GFRA / GDNFRa acts as the ligand-binding component. GDNF, a distantly related member of the transforming growth factor-beta (TGF-) superfamily, and its receptor components: GFRA1, Ret and neural cell adhesion molecule (NCAM) have been recently reported to be expressed in the testis and to be involved in the proliferation regulation of immature Sertoli cells.

ncbi gi num :

2624963

ncbi acc num :

AAB86600.1

ncbi mol weight :

Molecular Mass: 46.3 kDa. Actual Protein Molecular Mass: 55-60 kDa

ncbi pathways :

ARMS-mediated Activation Pathway (1366978); Axon Guidance Pathway (1366709); Cytokine Signaling In Immune System Pathway (1367586); DAP12 Interactions Pathway (1367572); DAP12 Signaling Pathway (1367573); Developmental Biology Pathway (1366708); Diurnally Regulated Genes With Circadian Orthologs Pathway (198406); Downstream Signal Transduction Pathway (1366988); Exercise-induced Circadian Regulation Pathway (198298); FCERI Mediated MAPK Activation Pathway (1367575)

ncbi summary :

This gene encodes a transmembrane protein that functions as the receptor for glial cell line derived neurotrophic factor (GDNF). The encoded protein undergoes proteolytic processing to generate a glycosylphosphatidylinositol-anchored cell surface coreceptor that forms a complex with the Ret tyrosine kinase in GDNF signaling pathway. Mice lacking the encoded protein exhibit deficits in the kidneys, the enteric nervous system, and spinal motor and sensory neurons similar mice deficient in GDNF or Ret. [provided by RefSeq, Jul 2016]

uniprot summary :

GFRA1: Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor. Belongs to the GDNFR family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, GPI anchor; Receptor, misc. Chromosomal Location of Human Ortholog: 19 D2 19 54.18 cM. Cellular Component: axon; cell soma; external side of plasma membrane; extracellular space; extrinsic to membrane; receptor complex. Molecular Function: integrin binding; receptor activity. Biological Process: anatomical structure morphogenesis; cell migration; nervous system development; neurite development; neuron differentiation; transmembrane receptor protein tyrosine kinase signaling pathway

size1 :

0.05 mg

price1 :

435 USD

size2 :

0.1 mg

price2 :

735

more info or order :

MyBioSource product webpage