protein

Recombinant Mouse SHP2 / PTPN11 Protein (His tag)

MBS2546971

0.1 mg

405 USD

Recombinant Protein

Recombinant Mouse SHP2 / PTPN11 Protein (His tag)

[SHP2 / PTPN11]

[PTPN11]

[tyrosine-protein phosphatase non-receptor type 11 isoform b; Tyrosine-protein phosphatase non-receptor type 11; tyrosine-protein phosphatase non-receptor type 11; protein tyrosine phosphatase, non-receptor type 11; Protein-tyrosine phosphatase SYP; SH-PTP2; SHP-2; Shp2]

[SHP2 / PTPN11]

[Ptpn11; Ptpn11; Syp; Shp2; PTP1D; PTP2C; SAP-2; SHP-2; SH-PTP2; SH-PTP3; AW536184; 2700084A17Rik; SHP-2; Shp2]

Human Cells

593

> 90 % as determined by SDS-PAGE

Lyophilized from sterile PBS, pH 7.4

Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted and be used as soon as possible. Avoid repeated freeze-thaw cycles.

The recombinant mouse PTPN11 comprises 604 amino acids and has a predicted molecular mass of 69.5 kDa. The apparent molecular mass of the protein is approximately 65 kDa in SDS-PAGE under reducing conditions due to glycosylation.

SDS-PAGE

Source: Mouse. Predicted N Terminal: Met

Endotoxin: 1 pmoles/min/ug. Molecular Mass: The recombinant mouse PTPN11 comprises 604 amino acids and has a predicted molecular mass of 69.5 kDa. The apparent molecular mass of the protein is approximately 65 kDa in SDS-PAGE under reducing conditions due to glycosylation. Reconstitution: It is recommended that sterile water (1000 ul) be added to the vial to prepare a stock solution of 0.1 mg/mL. Concentration is measured by UV-Vis.

Background: SHP2, also known as PTPN11, belongs to the protein-tyrosine phosphatase (PTP) family, non-receptor class 2 subfamily. PTPs catalyze the removal of phosphate groups from tyrosine residues by the hydrolysis of phosphoric acid monoesters. They dephosphorylate EGFR, JAK2 and TYK2 kinases, promoting oncogenic transformation. SHP2 is widely expressed, with highest levels in heart, brain, and skeletal muscle. SHP2 acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. It also dephosphorylates ROCK2 at Tyr-722 resulting in stimulatation of its RhoA binding activity. Description: A DNA sequence encoding the mouse PTPN11 (P35235-2) (Met1-Arg593) was expressed with a C-terminal polyhistidine tag.

158508568

NP_001103462.1

NM_001109992.1

P35235

68,035 Da

Adaptive Immune System Pathway (575096); Adipocytokine Signaling Pathway (83290); Adipocytokine Signaling Pathway (505); Axon Guidance Pathway (575211); B Cell Receptor Signaling Pathway (198285); CTLA4 Inhibitory Signaling Pathway (575106); Cell Surface Interactions At The Vascular Wall Pathway (575507); Chronic Myeloid Leukemia Pathway (83309); Chronic Myeloid Leukemia Pathway (528); Costimulation By The CD28 Family Pathway (575102)

SHP-2: a SH2-containing a ubiquitously expressed tyrosine-specific protein phosphatase. It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens and extracellular matrices in the control of cell growth, differentiation, migration, and death. Activation of SHP-2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines. Protein type: EC 3.1.3.48; Motility/polarity/chemotaxis; Protein phosphatase, tyrosine (non-receptor). Chromosomal Location of Human Ortholog: 5 5 F. Cellular Component: cell junction; cytoplasm; cytosol; membrane; mitochondrion; nucleus; protein complex; stress fiber. Molecular Function: cell adhesion molecule binding; D1 dopamine receptor binding; insulin receptor binding; insulin receptor substrate binding; non-membrane spanning protein tyrosine phosphatase activity; peptide hormone receptor binding; phospholipase binding; phosphoprotein phosphatase activity; protein binding; protein domain specific binding; protein tyrosine phosphatase activity; receptor tyrosine kinase binding; SH3/SH2 adaptor activity. Biological Process: abortive mitotic cell cycle; activation of MAPK activity; axonogenesis; Bergmann glial cell differentiation; brain development; cerebellar cortex formation; DNA damage checkpoint; ephrin receptor signaling pathway; epidermal growth factor receptor signaling pathway; genitalia development; glucose homeostasis; heart development; homeostasis of number of cells within a tissue; hormone metabolic process; hormone-mediated signaling; inner ear development; integrin-mediated signaling pathway; lipid metabolic process; microvillus organization and biogenesis; multicellular organism growth; negative regulation of cell adhesion mediated by integrin; negative regulation of cortisol secretion; negative regulation of growth hormone secretion; negative regulation of hormone secretion; negative regulation of insulin secretion; nerve growth factor receptor signaling pathway; organ growth; platelet formation; platelet-derived growth factor receptor signaling pathway; positive regulation of hormone secretion; positive regulation of mitotic cell cycle; positive regulation of signal transduction; protein amino acid dephosphorylation; regulation of cell adhesion mediated by integrin; regulation of multicellular organism growth; regulation of protein complex assembly; regulation of protein export from nucleus; reproductive process in a multicellular organism; triacylglycerol metabolic process

0.1 mg

405 USD

0.2 mg

635