protein

Recombinant Mouse PSGL-1 / CD162 / SELPLG Protein (Fc tag)

MBS2546795

0.05 mg

405 USD

Recombinant Protein

Recombinant Mouse PSGL-1 / CD162 / SELPLG Protein (Fc tag)

[PSGL-1 / CD162 / SELPLG]

[CD162, Psgl-1, Psgl1, Selp1, Selpl]

[P-selectin glycoprotein ligand 1; P-selectin glycoprotein ligand 1; P-selectin glycoprotein ligand 1; selectin, platelet (p-selectin) ligand; Selectin P ligand; CD_antigen: CD162]

[PSGL-1 / CD162 / SELPLG]

[Selplg; Selplg; CD162; Psgl1; Selp1; Selpl; Psgl-1; Selp1; Selpl; PSGL-1]

Human Cells.

397

> 85 % as determined by SDS-PAGE

Lyophilized from sterile PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% Tween 80

Store it under sterile conditions at -20°C to -80°C . It is recommended that the protein be aliquoted and be used as soon as possible. Avoid repeated freeze-thaw cycles.

The secreted recombinant mouse SELPLG/Fc is a disulfide-linked homodimer. The reduced monomer comprises 531 amino acids and has a calculated molecular mass of 57.4 kDa. As a result of glycosylation, the apparent molecular mass of rmSELPLG/Fc monomer is approximately 82 kDa in SDS-PAGE under reducing conditions.

SDS-PAGE

Source: Mouse. Predicted N Terminal: Leu 18. Protein Construction: A DNA sequence encoding the extracellular domain of mouse SELPLG (Q62170) (Met 1-Cys 307) was fused with the Fc region of human IgG1 at the C-terminus.

Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method. Molecular Mass: The secreted recombinant mouse SELPLG /Fc is a disulfide-linked homodimer. The reduced monomer comprises 531 amino acids and has a calculated molecular mass of 57.4 kDa. As a result of glycosylation, the apparent molecular mass of rmSELPLG/Fc monmer is approximately 82. Reconstitution: It is recommended that sterile water(200uL) be addded to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is measured by UV- Vis. Stability: Samples are stable for up to twelve months from date of receipt at -70°C

P-selectin glycoprotein ligand-1 (PSGL-1), also known as SELPLG or CD162, is the high affinitycounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 is a mucin-type glycoprotein, expressed on leukocytes and platelets as a homodimer of two disulfide-linked subunits of ~120 kD. As cell adhesion molecules, multiple studies have shown that PSGL-1/ P-selectin interaction is required for the normal recruitment of leukocytes during inflammatory reactions, and also participates in hemostatic responses. PSGL-1 protein requires two distinct posttranslational modifications for the Ca2+-dependent recognition by the lectin domain of P-selectin, that is tyrosine sulfation and specific O-linked glycosylation (sialic acid and fucose). PSGL-1 can also bind to other two members of the selectin family, E-selectin (endothelial) and L-selectin (leukocyte), but binds best to P-selectin. Description: A DNA sequence encoding the extracellular domain of mouse SELPLG (Q62170) (Met 1-Cys 307) was fused with the Fc region of human IgG1 at the C-terminus.

341942014

Q62170.2

Q62170

Cell Adhesion Molecules (CAMs) Pathway (83266); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (1368163); Hemostasis Pathway (1368135); Staphylococcus Aureus Infection Pathway (172855); Staphylococcus Aureus Infection Pathway (171867)

SELPLG: A SLe(x)-type glycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 5 F 5 55.59 cM. Cellular Component: plasma membrane; uropod. Molecular Function: protein binding. Biological Process: leukocyte adhesive activation; leukocyte tethering or rolling

0.05 mg

405 USD

0.1 mg

635