protein

Recombinant Mouse ROBO4 Protein (His tag)

MBS2546674

0.05 mg

405 USD

Recombinant Protein

Recombinant Mouse ROBO4 Protein (His tag)

[ROBO4]

[1200012D01Rik, AI593217, ECSM4, MRB]

[roundabout homolog 4 isoform 2; Roundabout homolog 4; roundabout homolog 4; roundabout guidance receptor 4]

[ROBO4]

[Robo4; Robo4; AI593217; 1200012D01Rik]

1015

> 97 % as determined by SDS-PAGE

Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% mannitol, 0.01% Tween-80

Store under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted and be used as soon as possible. Avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Source: Mouse. Expression Host: Human cells. Predicted Nterminal: Leu 38. Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method. Molecular Mass: The secreted recombinant mouse ROBO4 (aa 1-480) comprises 454 amino acids and has a calculated molecular mass of 49.6 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 65 kDa in SDS-PAGE under reducing conditions. Reconstitution: It is recommended that sterile water (330ul) be added to the vial to prepare a stock solution of 0.3 mg/ml. Concentration is measured by UV-Vis. Protein Construction: A DNA sequence encoding the mouse ROBO4 (NP_083059.2) N-terminal fragment (Met 1-Glu 480) was expressed, with a C-terminal polyhistidine tag.

Roundabout homolog 4, also known as magic roundabout and ROBO4 is a member of the immunoglobulin superfamily and ROBO family. ROBO4 is specifically expressed in endothelial cells. It is expressed at sites of angiogenesis in different tumor type s. ROBO4 contains two fibronectin type -III domains and two Ig-like C2-type (immunoglobulin-like) domains. ROBO4 is the fourth identified member of the roundabout receptor family. It is the only Robo family member expressed in primary endothelial cells and that application of Slit inhibits their migration. ROBO4 is predominantly expressed in embryonic or tumor vascular endothelium and is considered important for vascular development and as a candidate tumor endothelial marker. ROBO4 is a bona fide member of the Robo family and may provide a repulsive cue to migrating endothelial cells during vascular development. ROBO4 is a receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. ROBO4 may mediate the inhibition of primary endothelial cell migration by Slit proteins. Activating ROBO4 may have broad therapeutic application in diseases characterized by excessive angiogenesis and/or vascular leak.

27229187

NP_083059.2

NM_028783.3

Q8C310

ROBO4: Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins. Belongs to the immunoglobulin superfamily. ROBO family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 9 9 A4. Cellular Component: external side of plasma membrane; integral to membrane. Molecular Function: protein binding; receptor activity. Biological Process: angiogenesis; negative regulation of cell migration; regulation of apoptosis

0.05 mg

405 USD

0.1 mg

635