protein

Recombinant Human HER3 / ErbB3 Protein (His tag)

MBS2546418

0.01 mg

435 USD

Recombinant Protein

Recombinant Human HER3 / ErbB3 Protein (His tag)

[HER3 / ErbB3]

[HER3, LCCS2, ErbB-3, c-erbB3, erbB3-S, MDA-BF-1, MGC88033, c-erbB-3, p180-ErbB3, p45-sErbB3, p85-sErbB3]

[receptor tyrosine-protein kinase erbB-3 isoform 1; Receptor tyrosine-protein kinase erbB-3; receptor tyrosine-protein kinase erbB-3; erb-b2 receptor tyrosine kinase 3; Proto-oncogene-like protein c-ErbB-3; Tyrosine kinase-type cell surface receptor HER3]

[HER3 / ErbB3]

[ERBB3; ERBB3; HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3; HER3]

Human Cells

1342

> 95 % as determined by SDS-PAGE

Lyophilized from sterile PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% Tween80.

Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted and be used as soon as possible. Avoid repeated freeze-thaw cycles.

The recombinant human ErbB3 protein consists of 635 amino acids after the removal of signal peptide and has a calculated molecular mass of 70.2 kDa. As a result of glycosylation, the recombinant ErbB3 migrates as an approximately 100-110 kDa band in SDS-PAGE under reducing conditions.

SDS-PAGE

Source: Human. Predicted N Terminal: Ser 20. Bio-Activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized human ErbB3 at 2 ug/mL (100 ul/well) can bind human NRG1 (isoform Beta1), The EC50 of human NRG1 (isoform Beta1) is 0.43 ug/mL.

Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method. Reconstitution: It is recommended that sterile water (70 ul) be added to the vial to prepare a stock solution of 0.15 mg/mL. Concentration is measured by UV-Vis.

Background: ErbB3, also known as Her3 (human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form. Description: A DNA sequence encoding the extracellular domain (Met 1-Thr 643) of human ErbB3 (NP_001973.2) precursor was expressed, with a C-terminal polyhistidine tag.

54792100

NP_001973.2

NM_001982.3

P21860

77,426 Da

Adaptive Immune System Pathway (366160); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Disease Pathway (530764); Downregulation Of ERBB2:ERBB3 Signaling Pathway (530740); Downstream Signal Transduction Pathway (106385); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (576250)

This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

HER3: a receptor tyrosine kinase of the EGFR family. Binds and is activated by neuregulins and NTAK. Can form homodimers or ErbB-2/ErbB-3 heterodimers. Kinase domain lacks activity but heterodimerizes with other EGFRs to transduce growth signals. May be required for HER2 activity. Elevated expression in breast and other tumors is indicative of poor outcome. A secreted form is expressed in metastatic prostate cancer Two alternatively spliced isoforms have been described. Protein type: EC 2.7.10.1; EGFR family; Kinase, protein; Membrane protein, integral; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group. Chromosomal Location of Human Ortholog: 12q13.2. Cellular Component: basolateral plasma membrane; extracellular space; integral to plasma membrane; plasma membrane; receptor complex. Molecular Function: growth factor binding; identical protein binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; protein heterodimerization activity; protein tyrosine kinase activator activity; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; transmembrane receptor activity. Biological Process: cranial nerve development; heart development; MAPKKK cascade; negative regulation of cell adhesion; negative regulation of neuron apoptosis; negative regulation of secretion; negative regulation of signal transduction; neuron apoptosis; peripheral nervous system development; phosphoinositide 3-kinase cascade; phosphoinositide-mediated signaling; positive regulation of phosphoinositide 3-kinase cascade; regulation of cell proliferation; regulation of phosphoinositide 3-kinase cascade; Schwann cell differentiation; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway. Disease: Lethal Congenital Contracture Syndrome 2

0.01 mg

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